ArticlePloS one2013
Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 60 citations in OpenAlex.
- How Actin Polymerization and Myosin II Activity Regulate Focal Adhesion Dynamics in Motile Cells.International journal of molecular sciences · 2025Article
- G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.Cell reports · 2025Article
- YAP phosphorylation within integrin adhesions: Insights from a computational model.Biophysical journal · 2024Article
- Focal adhesions are controlled by microtubules through local contractility regulation.The EMBO journal · 2024Article
- Force-dependent focal adhesion assembly and disassembly: A computational study.PLoS computational biology · 2023Article
- Allosteric activation of vinculin by talin.Nature communications · 2023Article
- Article
- Organization, dynamics and mechanoregulation of integrin-mediated cell-ECM adhesions.Nature reviews. Molecular cell biology · 2023Review
- SFAlab: image-based quantification of mechano-active ventral actin stress fibers in adherent cells.Frontiers in cell and developmental biology · 2023Article
- VASP localization to lipid bilayers induces polymerization driven actin bundle formation.Molecular biology of the cell · 2022Article
- Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation.eLife · 2022Article
- A Layered View on Focal Adhesions.Biology · 2021Review
- Towards understanding the messengers of extracellular space: Computational models of outside-in integrin reaction networks.Computational and structural biotechnology journal · 2021Review
- Article
- Cellular contractile forces are nonmechanosensitive.Science advances · 2020Article
- Protein kinase A activity is regulated by actomyosin contractility during cell migration and is required for durotaxis.Molecular biology of the cell · 2020Article
- Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.Scientific reports · 2019Article
- Steps in Mechanotransduction Pathways that Control Cell Morphology.Annual review of physiology · 2019Review
- Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration.Biophysical journal · 2018Article
- Diverse patterns of molecular changes in the mechano-responsiveness of focal adhesions.Scientific reports · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Treatment of cultured cells with inhibitors of actomyosin contractility induces rapid deterioration of stress fibers, and disassembly of the associated focal adhesions (FAs). In this study, we show that treatment with the Rho kinase inhibitor Y-27632, which blocks actomyosin contractility, induces disarray in the FA-associated actin bundles, followed by the differential dissociation of eight FA components from the adhesion sites. Live-cell microscopy indicated that the drug triggers rapid dissociation of VASP and zyxin from FAs (τ values of 7-8 min), followed by talin, paxillin and ILK (τ ~16 min), and then by FAK, vinculin and kindlin-2 (τ = 25-28 min). Examination of the molecular kinetics of the various FA constituents, using Fluorescence Recovery After Photobleaching (FRAP), in the absence of or following short-term treatment with the drug, revealed major changes in the kon and koff values of the different proteins tested, which are in close agreement with their differential dissociation rates from the adhesion sites. These findings indicate that mechanical, actomyosin-generated forces differentially regulate the molecular kinetics of individual FA-associated molecules, and thereby modulate FA composition and stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.