ArticlePloS one2013
Impact of Helicobacter pylori biofilm formation on clarithromycin susceptibility and generation of resistance mutations.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 93 citations in OpenAlex.
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- Pathogenicity and virulence ofVirulence · 2025Review
- Forging biofilms: metal-induced microbial responses in biofilm formation.Journal of bacteriology · 2025Review
- Antibiotic resistance in Helicobacter pylori: a genetic and physiological perspective.Gut pathogens · 2025Review
- Nanomaterials: A Prospective Strategy for Biofilm-FormingInternational journal of nanomedicine · 2025Review
- Review
- Molecular Mechanisms of Biofilm Formation inAntibiotics (Basel, Switzerland) · 2024Review
- Opportunities forMicroorganisms · 2024Review
- Effect and mechanism of Qingre Huashi decoction on drug-resistantWorld journal of gastroenterology · 2024Article
- Molecular tools of antibiotic resistance forFrontiers in gastroenterology (Lausanne, Switzerland) · 2024Review
- Biofilm and Cancer: Interactions and Future Directions for Cancer Therapy.International journal of molecular sciences · 2023Review
- Genetic determinants of Biofilm formation of Helicobacter pylori using whole-genome sequencing.BMC microbiology · 2023Article
- Discovery of Novel Resistance Mechanisms ofAntibiotics (Basel, Switzerland) · 2023Article
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- Multi-functional vesicles improveActa pharmaceutica Sinica. B · 2022Article
- In Vitro Activity of the Arylaminoartemisinin GC012 againstPathogens (Basel, Switzerland) · 2022Article
- Review
- Prevalence of antibiotic resistance ofAmerican journal of translational research · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human gastric pathogen Helicobacter pylori forms biofilms in vitro and in vivo. The purpose of this study was to evaluate the effects of H. pylori biofilm formation in vitro on clarithromycin (CLR) susceptibility. CLR susceptibility of H. pylori intermediate (2-day) and mature (3-day) biofilms on glass coverslips was determined at concentrations from 0.03 to 0.5 µg/ml. H. pylori biofilm biomass was increased after treatment with CLR at minimum inhibitory concentration levels by up to 4-fold (2-day biofilm) and 16-fold (3-day biofilm). Minimum bactericidal concentrations of CLR against cells in a biofilm were higher (1.0 µg/ml) than that for planktonic cells (0.25 µg/ml). It was shown that the expression of efflux pump genes was significantly increased in biofilm cells. In addition, exposure of biofilms to CLR resulted in high level resistance generation compared to planktonic cells with increased resistance associated with the presence of a point mutation at either position 2142 or 2143 in the domain V loop of the 23S rRNA gene. These results demonstrate that H. pylori biofilm formation decreases the susceptibility to CLR and that H. pylori CLR resistance mutations are more frequently generated in biofilms than in planktonic cells.
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