ArticlePloS one2013
Targeted biomarker discovery by high throughput glycosylation profiling of human plasma alpha1-antitrypsin and immunoglobulin A.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- In-depth plasma N-glycoproteome profiling using narrow-window data-independent acquisition on the Orbitrap Astral mass spectrometer.Nature communications · 2025Article
- O-glycosylation of IgA1 and the pathogenesis of an autoimmune disease IgA nephropathy.Glycobiology · 2024Review
- The genetics and epidemiology of N- and O-immunoglobulin A glycomics.Genome medicine · 2024Article
- Altered Serum Alpha1-Antitrypsin Protease Inhibition before and after Clinical Hematopoietic Stem Cell Transplantation: Association with Risk for Non-Relapse Mortality.International journal of molecular sciences · 2023Article
- Quantitative clinical glycomics strategies: A guide for selecting the best analysis approach.Mass spectrometry reviews · 2022Review
- Glycosylation Biomarkers Associated with Age-Related Diseases and Current Methods for Glycan Analysis.International journal of molecular sciences · 2021Review
- HILIC-UPLC-MS for high throughput and isomeric N-glycan separation and characterization in Congenital Disorders Glycosylation and human diseases.Glycoconjugate journal · 2021Article
- Meta-heterogeneity: Evaluating and Describing the Diversity in Glycosylation Between Sites on the Same Glycoprotein.Molecular & cellular proteomics : MCP · 2021Review
- Glycosylation and Aging.Advances in experimental medicine and biology · 2021Article
- Glycomics Microarrays Reveal Differential In Situ Presentation of the Biofilm Polysaccharide Poly-International journal of molecular sciences · 2020Article
- Antibody Panel Based N-Glycan Imaging for N-Glycoprotein Biomarker Discovery.Current protocols in protein science · 2019Article
- Immunoglobulin A N-glycosylation Presents Important Body Fluid-specific Variations in Lactating Mothers.Molecular & cellular proteomics : MCP · 2019Article
- High-throughput SerumMolecular & cellular proteomics : MCP · 2019Article
- Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM.Journal of proteomics · 2018Article
- Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.Chemical reviews · 2018Review
- The Continuum of Aging and Age-Related Diseases: Common Mechanisms but Different Rates.Frontiers in medicine · 2018Review
- Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.Analytical and bioanalytical chemistry · 2017Article
- Human Plasma N-glycosylation as Analyzed by Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance-MS Associates with Markers of Inflammation and Metabolic Health.Molecular & cellular proteomics : MCP · 2017Article
- Recent Advances in the Analysis of Complex Glycoproteins.Analytical chemistry · 2017Review
- N-Glycan profile analysis of transferrin using a microfluidic compact disc and MALDI-MS.Analytical and bioanalytical chemistry · 2016Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein N-glycosylation patterns are known to show vast genetic as well as physiological and pathological variation and represent a large pool of potential biomarkers. Large-scale studies are needed for the identification and validation of biomarkers, and the analytical techniques required have recently been developed. Such methods have up to now mainly been applied to complex mixtures of glycoproteins in biofluids (e.g. plasma). Here, we analyzed N-glycosylation profiles of alpha1-antitrypsin (AAT) and immunoglobulin A (IgA) enriched fractions by 96-well microtitration plate based high-throughput immuno-affinity capturing and N-glycan analysis using multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (CGE-LIF). Human plasma samples were from the Leiden Longevity Study comprising 2415 participants of different chronological and biological ages. Glycosylation patterns of AAT enriched fractions were found to be associated with chronological (calendar) age and they differed between females and males. Moreover, several glycans in the AAT enriched fraction were associated with physiological parameters marking cardiovascular and metabolic diseases. Pronounced differences were found between males and females in the glycosylation profiles of IgA enriched fractions. Our results demonstrate that large-scale immuno-affinity capturing of proteins from human plasma using a bead-based method combined with high-throughput N-glycan analysis is a powerful tool for the discovery of glycosylation-based biomarker candidates.
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