ArticlePloS one2013
Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- AUP1 and UBE2G2 complex targets STING signaling and regulates virus-induced innate immunity.mBio · 2025Article
- LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings.The Plant cell · 2025Article
- Cell-intrinsic insulin signaling defects in human iPS cell-derived hepatocytes in type 2 diabetes.The Journal of clinical investigation · 2025Article
- A novel bacterial effector protein mediates ER-LD membrane contacts to regulate host lipid droplets.EMBO reports · 2024Article
- AUP1 regulates lipid metabolism and induces lipid accumulation to accelerate the progression of renal clear cell carcinoma.Cancer science · 2022Article
- A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2 and is essential for ER-associated degradation.PLoS biology · 2021Article
- Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells.Autophagy · 2021Article
- A Decade of Mighty Lipophagy: What We Know and What Facts We Need to Know?Oxidative medicine and cellular longevity · 2021Review
- Article
- Dynamics and functions of lipid droplets.Nature reviews. Molecular cell biology · 2019Review
- Lipid droplet functions beyond energy storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Breaking fat: The regulation and mechanisms of lipophagy.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Establishing the lipid droplet proteome: Mechanisms of lipid droplet protein targeting and degradation.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- A Luciferase-fragment Complementation Assay to Detect Lipid Droplet-associated Protein-Protein Interactions.Molecular & cellular proteomics : MCP · 2017Article
- Modulation of Lipid Droplet Metabolism-A Potential Target for Therapeutic Intervention inFrontiers in microbiology · 2017Review
- Autophagy in the liver: cell's cannibalism and beyond.Archives of pharmacal research · 2016Review
- Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells.Plant signaling & behavior · 2013Article
- ER-LD Membrane Contact Sites: A Budding Area in the Pathogen Survival Strategy.Contact (Thousand Oaks (Ventura County, Calif.))Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid droplets, the intracellular storage organelles for neutral lipids, exist in a wide range of sizes and of morphologically distinct organization, from loosely dispersed lipid droplets to tightly packed lipid droplet clusters. We show that the lipid droplet protein AUP1 induces cluster formation. A fraction of AUP1 is monoubiquitinated at various lysine residues. This process depends on its internal CUE domain, which is a known ubiquitin-binding domain. AUP1 with a deleted or point mutagenized CUE domain, as well as a lysine-free mutant, are not ubiquitinated and do not induce lipid droplet clustering. When such ubiquitination deficient mutants are fused to ubiquitin, clustering is restored. AUP1 mutants with defective droplet targeting fail to induce clustering. Also, another lipid droplet protein, NSDHL, with a fused ubiquitin does not induce clustering. The data indicate that monoubiquitinated AUP1 on the lipid droplet surface specifically induces clustering, and suggest a homophilic interaction with a second AUP1 molecule or a heterophilic interaction with another ubiquitin-binding protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.