ArticlePLoS pathogens2013
The zebrafish as a new model for the in vivo study of Shigella flexneri interaction with phagocytes and bacterial autophagy.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers, 2 of them syntheses that pooled it.
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Who cites it
108 citing papers in PubMed, 2 syntheses or guidelines pooled it, 191 citations in OpenAlex.
- Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy · 2021Guideline
- Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).Autophagy · 2016Guideline
- Proximity biotinylation at the host-Shigella interface reveals UFMylation as an antibacterial pathway.Nature communications · 2026Article
- Premna odorata extract exhibits anti-inflammatory activity via suppression of NLRP3 inflammasome.Scientific reports · 2026Article
- TLR7/8 agonist 3M-052 formulated in micelles induces local type I IFN response and antiviral immunity in zebrafish larvae.Frontiers in immunology · 2026Article
- Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei.Nature communications · 2025Article
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- Organoid models advance dysentery control.Nature genetics · 2025Article
- Proximity biotinylation at the host-bioRxiv : the preprint server for biology · 2025Article
- An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae.Zebrafish · 2025Article
- Autophagy in aquatic animals: mechanisms, implications, and future directions.Frontiers in immunology · 2025Review
- The battle between bacterial infection and autophagy in aquatic animals.Frontiers in immunology · 2025Review
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- An Image Processing Tool for Automated Quantification of Bacterial Burdens in Zebrafish Larvae.bioRxiv : the preprint server for biology · 2024Article
- Epithelial Cell Infection Analyses with Shigella.Journal of visualized experiments : JoVE · 2024Article
- Transcriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri.Disease models & mechanisms · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Shigella Serotypes Associated With Carriage in Humans Establish Persistent Infection in Zebrafish.The Journal of infectious diseases · 2023Article
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- Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
Autophagy, an ancient and highly conserved intracellular degradation process, is viewed as a critical component of innate immunity because of its ability to deliver cytosolic bacteria to the lysosome. However, the role of bacterial autophagy in vivo remains poorly understood. The zebrafish (Danio rerio) has emerged as a vertebrate model for the study of infections because it is optically accessible at the larval stages when the innate immune system is already functional. Here, we have characterized the susceptibility of zebrafish larvae to Shigella flexneri, a paradigm for bacterial autophagy, and have used this model to study Shigella-phagocyte interactions in vivo. Depending on the dose, S. flexneri injected in zebrafish larvae were either cleared in a few days or resulted in a progressive and ultimately fatal infection. Using high resolution live imaging, we found that S. flexneri were rapidly engulfed by macrophages and neutrophils; moreover we discovered a scavenger role for neutrophils in eliminating infected dead macrophages and non-immune cell types that failed to control Shigella infection. We observed that intracellular S. flexneri could escape to the cytosol, induce septin caging and be targeted to autophagy in vivo. Depletion of p62 (sequestosome 1 or SQSTM1), an adaptor protein critical for bacterial autophagy in vitro, significantly increased bacterial burden and host susceptibility to infection. These results show the zebrafish larva as a new model for the study of S. flexneri interaction with phagocytes, and the manipulation of autophagy for anti-bacterial therapy in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.