ArticleMeasurement science & technology2013
Dual channel detection of ultra low concentration of bacteria in real time by scanning FCS.
Article in Measurement science & technology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Digital Protein Detection in Bulk Solutions.ACS omega · 2022Article
- Bacterial Infection Diagnosis and Antibiotic Prescription in 3 h as an Answer to Antibiotic Resistance: The Case of Urinary Tract Infections.Antibiotics (Basel, Switzerland) · 2021Article
- Rapid Detection of β-Lactamase-Producing Bacteria Using the Integrated Comprehensive Droplet Digital Detection (IC 3D) System.Sensors (Basel, Switzerland) · 2020Article
- Rapid isolation of rare targets from large fluid volumes.Scientific reports · 2020Article
- Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.Chemical science · 2020Review
- Scanning single-molecule counting system for Eprobe with highly simple and effective approach.PloS one · 2020Article
- A rapid, point-of-care antibiotic susceptibility test for urinary tract infections.Journal of medical microbiology · 2020Article
- Fluorescence lifetime detection with particle counting devices.Biomedical optics express · 2019Article
- A Fluorescence Correlation Spectrometer for Measurements in Cuvettes.Biophysical journal · 2018Article
- Back to the Future: Fluorescence Correlation Spectroscopy Moves Back in the Cuvette.Biophysical journal · 2018Article
- Emerging Technologies for Molecular Diagnosis of Sepsis.Clinical microbiology reviews · 2018Review
- Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection.Lab on a chip · 2015Article
- Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection.Nature communications · 2014Article
- Simplified confocal microscope for counting particles at low concentrations.The Review of scientific instruments · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
We describe a novel method to detect very low concentrations of bacteria in water. Our device consists of a portable horizontal geometry small confocal microscope with large pinhole and a holder for cylindrical cuvettes containing the sample. Two motors provide a fast rotational and slow vertical motion of the cuvette so the device looks like a simplified flow cytometer without flow. To achieve high sensitivity the design has two detection channels. Bacteria are stained by two different nucleic acid dyes and excited with two different lasers. Data are analyzed with a correlation filter based on particle passage pattern recognition. The passage of a particle through the illumination volume is compared with a Gaussian pattern in both channels. The width of the Gaussian correlates with the time of passage of the particle so one particle is counted when the algorithm finds a match with a Gaussian in both channels. The concentration of particles in the sample is deduced from the total number of coincident hits and the total volume scanned. This portable setup provides higher sensitivity, low cost and it could have a wide use ranging from clinical applications to pollution monitors and water and air quality control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.