ArticleJournal of bacteriology2013
VhuD facilitates electron flow from H2 or formate to heterodisulfide reductase in Methanococcus maripaludis.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Recent advances in exploring the composition and evolution of the prokaryotic selenoproteome.Applied and environmental microbiology · 2026Review
- Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- Article
- Functionally redundant formate dehydrogenases enable formate-dependent growth in Methanococcus maripaludis.The Journal of biological chemistry · 2024Article
- Hydrogenotrophic methanogenesis is the key process in the obligately syntrophic consortium of the anaerobic ameba Pelomyxa schiedti.The ISME journal · 2023Article
- Metabolic Strategies Shared by Basement Residents of the Lost City Hydrothermal Field.Applied and environmental microbiology · 2022Article
- The Fluorescence-Activating and Absorption-Shifting Tag (FAST) Enables Live-Cell Fluorescence Imaging of Methanococcus maripaludis.Journal of bacteriology · 2022Article
- Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea.PLoS biology · 2022Review
- Diversification of methanogens into hyperalkaline serpentinizing environments through adaptations to minimize oxidant limitation.The ISME journal · 2021Article
- Formate-Dependent Heterodisulfide Reduction in aApplied and environmental microbiology · 2021Article
- Putative Extracellular Electron Transfer in Methanogenic Archaea.Frontiers in microbiology · 2021Review
- Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer.Journal of the Royal Society, Interface · 2019Article
- Methanogens: pushing the boundaries of biology.Emerging topics in life sciences · 2018Article
- Origin and Evolution of Flavin-Based Electron Bifurcating Enzymes.Frontiers in microbiology · 2018Article
- Electron Bifurcation and Confurcation in Methanogenesis and Reverse Methanogenesis.Frontiers in microbiology · 2018Review
- Homoacetogenesis in Deep-SeamBio · 2017Article
- Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea.Applied and environmental microbiology · 2017Article
- Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments.The ISME journal · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Flavin-based electron bifurcation has recently been characterized as an essential energy conservation mechanism that is utilized by hydrogenotrophic methanogenic Archaea to generate low-potential electrons in an ATP-independent manner. Electron bifurcation likely takes place at the flavin associated with the α subunit of heterodisulfide reductase (HdrA). In Methanococcus maripaludis the electrons for this reaction come from either formate or H2 via formate dehydrogenase (Fdh) or Hdr-associated hydrogenase (Vhu). However, how these enzymes bind to HdrA to deliver electrons is unknown. Here, we present evidence that the δ subunit of hydrogenase (VhuD) is central to the interaction of both enzymes with HdrA. When M. maripaludis is grown under conditions where both Fdh and Vhu are expressed, these enzymes compete for binding to VhuD, which in turn binds to HdrA. Under these conditions, both enzymes are fully functional and are bound to VhuD in substoichiometric quantities. We also show that Fdh copurifies specifically with VhuD in the absence of other hydrogenase subunits. Surprisingly, in the absence of Vhu, growth on hydrogen still occurs; we show that this involves F420-reducing hydrogenase. The data presented here represent an initial characterization of specific protein interactions centered on Hdr in a hydrogenotrophic methanogen that utilizes multiple electron donors for growth.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.