ArticleThe EMBO journal1990
Characterization of the sequence-specific interaction of mouse c-myb protein with DNA.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 160 citations in OpenAlex.
- OsMYB14, an R2R3-MYB transcription factor, regulates plant height through the control of hormone metabolism in rice.Molecules and cells · 2024Article
- Insights into the Diversification and Evolution of R2R3-MYB Transcription Factors in Plants.Plant physiology · 2020Article
- A c-Myb mutant causes deregulated differentiation due to impaired histone binding and abrogated pioneer factor function.Nucleic acids research · 2017Article
- Trim33 Binds and Silences a Class of Young Endogenous Retroviruses in the Mouse Testis; a Novel Component of the Arms Race between Retrotransposons and the Host Genome.PLoS genetics · 2015Article
- MYT3, a Myb-like transcription factor, affects fungal development and pathogenicity of Fusarium graminearum.PloS one · 2014Article
- Quadruple 9-mer-based protein binding microarray analysis confirms AACnG as the consensus nucleotide sequence sufficient for the specific binding of AtMYB44.Molecules and cells · 2012Article
- Selective inhibition of c-Myb DNA-binding by RNA polymers.BMC biochemistry · 2004Article
- Long-range effects of retroviral insertion on c-myb: overexpression may be obscured by silencing during tumor growth in vitro.Journal of virology · 2003Article
- Article
- Interaction of murine BiP/GRP78 with the DnaJ homologue MTJ1.The Journal of biological chemistry · 2000Article
- Hyperphosphorylation and increased proteolytic breakdown of c-Myb induced by the inhibition of Ser/Thr protein phosphatases.Oncogene · 2000Article
- Transformation by v-Myb.Oncogene · 1999Review
- Review
- Mutations in the yeast Myb-like protein Bas1p resulting in discrimination between promoters in vivo but notin vitro.Nucleic acids research · 1998Article
- Sequence-specific DNA recognition by the myb-like domain of the human telomere binding protein TRF1: a model for the protein-DNA complex.Nucleic acids research · 1998Article
- Evidence for direct activation of an anthocyanin promoter by the maize C1 protein and comparison of DNA binding by related Myb domain proteins.The Plant cell · 1997Article
- A family of novel myb-related genes from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation.Plant molecular biology · 1996Article
- The importance of the linker connecting the repeats of the c-Myb oncoprotein may be due to a positioning function.Nucleic acids research · 1996Article
- Interaction of the co-activator CBP with Myb proteins: effects on Myb-specific transactivation and on the cooperativity with NF-M.The EMBO journal · 1996Article
- Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene.Molecular and cellular biology · 1996Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have examined parameters that affect sequence-specific interactions of the mouse c-myb protein with DNA oligomers containing the Myb-binding motif (CA/CGTTPu). Complexes formed between these oligomers and in vitro translated c-myb proteins were analysed by electrophoresis on non-denaturing polyacrylamide gels using the mobility-shift assay. By progressive truncation of c-myb coding sequences it was demonstrated that amino acids downstream of a region of three imperfect 51-52 residue repeats (designated R1, R2 and R3), which are located close to the amino terminus of the protein, had no qualitative or quantitative effect on the ability to interact specifically with this DNA motif. However, removal of only five amino acids of the R3 repeat completely abolished this activity. The contribution of individual DNA-binding domain repeats to this interaction was investigated by precisely deleting each individually: it was demonstrated that a combination of R2 and R3 was absolutely required for complex formation while the R1 repeat was completely dispensible. c-myb proteins showed quantitatively greater interaction with oligomers containing duplicated rather than single Myb-binding motif, in particular where these were arranged in tandem. Moreover, it was observed that c-myb protein interacted with these tandem motifs as a monomer. These findings imply that a single protein subunit straddles adjacent binding sites and the implications for c-myb activity are discussed.
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