ArticleNucleic acids research2013
The splicing landscape is globally reprogrammed during male meiosis.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 105 citations in OpenAlex.
- Minor spliceosome inhibition via ablation of its U11 snRNA gene results in multiple defects in murine spermatogenesis.Nucleic acids research · 2026Article
- PCID2 is essential for spermatogonial differentiation by regulating alternative splicing.Cellular and molecular life sciences : CMLS · 2026Article
- Ultra-Conserved Poison Exons Enable Rapid and Safe Splicing Factor Gene Expression Switches: A Hypothesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- CK1α in Sertoli cells is essential for testicular development and spermatogenesis in mice.Zoological research · 2025Article
- Single-cell RNA sequencing reveals the critical role of alternative splicing in cattle testicular spermatagonia.Biology direct · 2024Article
- PTBP1 mediates Sertoli cell actin cytoskeleton organization by regulating alternative splicing of actin regulators.Nucleic acids research · 2024Article
- CWF19L2 is Essential for Male Fertility and Spermatogenesis by Regulating Alternative Splicing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- PTBP1 mediates Sertoli cell actin cytoskeleton organization by regulating alternative splicing of actin regulators.bioRxiv : the preprint server for biology · 2024Article
- A Comparative Full-Length Transcriptome Analysis Using Oxford Nanopore Technologies (ONT) in Four Tissues of Bovine Origin.Animals : an open access journal from MDPI · 2024Article
- Uncovering a multitude of stage-specific splice variants and putative protein isoforms generated along mouse spermatogenesis.BMC genomics · 2024Article
- Alternative splicing and related RNA binding proteins in human health and disease.Signal transduction and targeted therapy · 2024Review
- Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling.eLife · 2024Article
- mRNA-Seq of testis and liver tissues reveals a testis-specific gene and alternative splicing associated with hybrid male sterility in dzo.Journal of animal science · 2024Article
- Purification by STA-PUT Technique of Male Germ Cells from Single Mouse and RNA-Extraction for Transcriptomic Analysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling.bioRxiv : the preprint server for biology · 2023Article
- An ancient testis-specific IQ motif-containing H gene regulates specific transcript isoform expression during spermatogenesis.Development (Cambridge, England) · 2023Article
- Article
- SRSF1-mediated alternative splicing is required for spermatogenesis.International journal of biological sciences · 2023Article
- Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation.Cell death and differentiation · 2023Article
- SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing.eLife · 2022Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Meiosis requires conserved transcriptional changes, but it is not known whether there is a corresponding set of RNA splicing switches. Here, we used RNAseq of mouse testis to identify changes associated with the progression from mitotic spermatogonia to meiotic spermatocytes. We identified ∼150 splicing switches, most of which affect conserved protein-coding exons. The expression of many key splicing regulators changed in the course of meiosis, including downregulation of polypyrimidine tract binding protein (PTBP1) and heterogeneous nuclear RNP A1, and upregulation of nPTB, Tra2β, muscleblind, CELF proteins, Sam68 and T-STAR. The sequences near the regulated exons were significantly enriched in target sites for PTB, Tra2β and STAR proteins. Reporter minigene experiments investigating representative exons in transfected cells showed that PTB binding sites were critical for splicing of a cassette exon in the Ralgps2 mRNA and a shift in alternative 5' splice site usage in the Bptf mRNA. We speculate that nPTB might functionally replace PTBP1 during meiosis for some target exons, with changes in the expression of other splicing factors helping to establish meiotic splicing patterns. Our data suggest that there are substantial changes in the determinants and patterns of alternative splicing in the mitotic-to-meiotic transition of the germ cell cycle.
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