ArticleTransgenic research2014
Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.
Article in Transgenic research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 117 citations in OpenAlex.
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- Involvement of a NIMA-related kinase in cell division of the liverwort Marchantia polymorpha.Plant & cell physiology · 2025Article
- Identification and characterization of DICER-LIKE genes and their roles in Marchantia polymorpha development and salt stress response.The Plant journal : for cell and molecular biology · 2025Article
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- Optimizing Promoters and Subcellular Localization for Constitutive Transgene Expression in Marchantia polymorpha.Plant & cell physiology · 2024Article
- The landscape of transcription factor promoter activity during vegetative development in Marchantia.The Plant cell · 2024Article
- The cAMP signaling module regulates sperm motility in the liverwortProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Biosynthesis of gibberellin-related compounds modulates far-red light responses in the liverwort Marchantia polymorpha.The Plant cell · 2023Article
- Auxin signaling is essential for organogenesis but not for cell survival in the liverwort Marchantia polymorpha.The Plant cell · 2023Article
- R2R3-MYB transcription factor GEMMA CUP-ASSOCIATED MYB1 mediates the cytokinin signal to achieve proper organ development in Marchantia polymorpha.Scientific reports · 2022Article
- The annotation and analysis of complex 3D plant organs using 3DCoordX.Plant physiology · 2022Article
- A versatile TnPlant communications · 2022Article
- Developmental Plasticity of the Amphibious LiverwortFrontiers in plant science · 2022Article
- Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the LiverwortInternational journal of molecular sciences · 2021Article
- An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.The New phytologist · 2021Article
- Article
- HAG1 and SWI3A/B control of male germ line development in P. patens suggests conservation of epigenetic reproductive control across land plants.Plant reproduction · 2021Article
- Characterization of a Plant Nuclear Matrix Constituent Protein in Liverwort.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Constitutive promoters are essential tools for analyses of gene functions by transgenic approaches. For overexpression and silencing studies of genes, a ubiquitous and strong expression of genes under investigation as well as selection markers is preferred. For future applications in the emerging basal plant model system Marchantia polymorpha, a liverwort, activities of the viral 35S cauliflower mosaic virus promoter and the endogenous elongation factor 1α (MpEF1α) promoter were analyzed. Expression of the reporter gene β-glucuronidase (GUS), driven by the CaMV35 and MpEF1α promoters, was compared throughout plant development. Significant differences were observed between the two promoter activities. The CaMV35 promoter yields a weak reporter gene expression in the meristematic zones but drives a strong expression in the thallus. The MpEF1α promoter causes a strong meristematic GUS expression and is more active in female sexual tissues. Overall, the MpEF1α promoter seems to be the better option for obtaining a strong and ubiquitous transgene expression. Furthermore, a whole mount in situ hybridization protocol for Marchantia was established. Analysis of MpEF1α mRNA transcript in intact, whole tissues showed an expression pattern that is overall similar to the pattern of the GUS reporter gene expression driven by the MpEF1α promoter, including strong expression in meristematic zones. The whole mount technique reported here can be used to determine the mRNA expression in intact gemmae and archegonia, and has the potential to be applied for screening large numbers of transgenic plants, for instance to identify knock-down mutants.
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