ArticleCell cycle (Georgetown, Tex.)2013
Laser microbeam-induced DNA damage inhibits cell division in fertilized eggs and early embryos.
Article in Cell cycle (Georgetown, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- The Consequences of DNA Damage in the Early Embryo Are Important for Practical Procedures in Assisted Reproduction.International journal of molecular sciences · 2025Review
- Article
- Cleavage of Early Mouse Embryo with Damaged DNA.International journal of molecular sciences · 2022Article
- ELF4 is critical to zygotic gene activation and epigenetic reprogramming during early embryonic development in pigs.Frontiers in veterinary science · 2022Article
- DNA damage repair is suppressed in porcine aged oocytes.Journal of animal science and technology · 2021Article
- Histone Lysine Demethylases KDM5B and KDM5C Modulate Genome Activation and Stability in Porcine Embryos.Frontiers in cell and developmental biology · 2020Article
- Label-free assessment of pre-implantation embryo quality by the Fluorescence Lifetime Imaging Microscopy (FLIM)-phasor approach.Scientific reports · 2019Article
- Live-cell imaging of nuclear-chromosomal dynamics in bovine in vitro fertilised embryos.Scientific reports · 2018Article
- RAD51 maintains chromosome integrity and mitochondrial distribution during porcine oocyte maturation in vitro.The Journal of reproduction and development · 2017Article
- The possible molecular mechanisms of bisphenol A action on porcine early embryonic development.Scientific reports · 2017Article
- Effect of ATM and HDAC Inhibition on Etoposide-Induced DNA Damage in Porcine Early Preimplantation Embryos.PloS one · 2015Article
- Effects of DNA damage and short-term spindle disruption on oocyte meiotic maturation.Histochemistry and cell biology · 2014Article
- Different fates of oocytes with DNA double-strand breaks in vitro and in vivo.Cell cycle (Georgetown, Tex.) · 2014Article
- Inhibition of histone deacetylases enhances DNA damage repair in SCNT embryos.Cell cycle (Georgetown, Tex.) · 2014Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA double-strand breaks are caused by both intracellular physiological processes and environmental stress. In this study, we used laser microbeam cut (abbreviated microcut or cut), which allows specific DNA damage in the pronucleus of a fertilized egg and in individual blastomere(s) of an early embryo, to investigate the response of early embryos to DNA double-strand breaks. Line type γH2AX foci were detected in the cut region, while Chk2 phosphorylation staining was observed in the whole nuclear region of the cut pronuclei or blastomeres. Zygotes with cut male or female pronucleus showed poor developmental capability: the percentage of cleavage embryos was significantly decreased, and the embryos failed to complete further development to blastocysts. The cut blastomeres in 2-cell, 4-cell, and 8-cell embryos ceased cleavage, and they failed to incorporate into compacted morulae, but instead underwent apoptosis and cell death at the blastocyst stage; the uncut part of embryos could develop to blastocysts, with a reduced percentage or decreased cell number. When both blastomeres of the 2-cell embryos were cut by laser microbeam, cell death occurred 24 h earlier, suggesting important functions of the uncut blastomere in delaying cell death of the cut blastomere. Taken together, we conclude that microbeam-induced DNA damage in early embryos causes compromised development, and that embryos may have their own mechanisms to exclude DNA-damaged blastomeres from participating in further development.
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