ArticleMolecular and cellular biology1990
Molecular and functional analysis of the muscle-specific promoter region of the Duchenne muscular dystrophy gene.
Article in Molecular and cellular biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 142 citations in OpenAlex.
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- Dp71 Point Mutations Induce Protein Aggregation, Loss of Nuclear Lamina Integrity and Impaired Braf35 and Ibraf Function in Neuronal Cells.International journal of molecular sciences · 2022Article
- Targeting IRES-dependent translation as a novel approach for treating Duchenne muscular dystrophy.RNA biology · 2021Review
- Single-transcript multiplexWellcome open research · 2020Article
- Identification and expression of SRF targeted by miR-133a during early development of Paralichthys olivaceus.Fish physiology and biochemistry · 2015Article
- Ordered disorder of the astrocytic dystrophin-associated protein complex in the norm and pathology.PloS one · 2013Article
- Article
- Identification of seven novel cryptic exons embedded in the dystrophin gene and characterization of 14 cryptic dystrophin exons.Journal of human genetics · 2007Article
- Bupivacaine hydrochloride induces muscle fiber necrosis and hydroxyl radical formation-dimethyl sulphoxide reduces hydroxyl radical formation.Neurochemical research · 2001Article
- Critical regulatory domains in intron 2 of a porcine sarcomeric myosin heavy chain gene.Journal of muscle research and cell motility · 2000Article
- A second promoter provides an alternative target for therapeutic up-regulation of utrophin in Duchenne muscular dystrophy.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The membrane-cytoskeleton interface: the role of dystrophin and utrophin.Journal of muscle research and cell motility · 1997Review
- A muscle-specific enhancer within intron 1 of the human dystrophin gene is functionally dependent on single MEF-1/E box and MEF-2/AT-rich sequence motifs.Nucleic acids research · 1997Article
- Serum response factor and protein-mediated DNA bending contribute to transcription of the dystrophin muscle-specific promoter.Molecular and cellular biology · 1997Article
- Expression and activity of serum response factor is required for expression of the muscle-determining factor MyoD in both dividing and differentiating mouse C2C12 myoblasts.Molecular biology of the cell · 1996Article
- Expression and regulation of the dystrophin Purkinje promoter in human skeletal muscle, heart, and brain.Human genetics · 1996Article
- Re-evaluation of the distributions of dystrophin and utrophin in sciatic nerve.The Biochemical journal · 1995Article
- A mutation in the dystrophin gene selectively affecting dystrophin expression in the heart.The Journal of clinical investigation · 1995Article
- Different muscle specific promoter characteristics in two sibs with Duchenne muscular dystrophy.Journal of medical genetics · 1995Article
- Transcription of the dystrophin gene in normal tissues and in skeletal muscle of a family with X-linked dilated cardiomyopathy.American journal of human genetics · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Duchenne muscular dystrophy (DMD) gene transcripts are most abundant in normal skeletal and cardiac muscle and accumulate as normal myoblasts differentiate into multinucleated myotubes. In this report we describe our initial studies aimed at defining the cis-acting sequences and trans-acting factors involved in the myogenic regulation of DMD gene transcription. A cosmid clone containing the first exon of the DMD gene has been isolated, and sequences lying upstream of exon 1 were analyzed for homologies to other muscle-specific gene promoters and for their ability to direct muscle-specific transcription of chimeric chloramphenicol acetyltransferase (CAT) gene constructs. The results indicate that the transcriptional start site for this gene lies 37 base pairs (bp) upstream of the 5' end of the published cDNA sequence and that 850 bp of upstream sequence can direct CAT gene expression in a muscle-specific manner. Sequence analysis indicates that in addition to an ATA and GC box, this region contains domains that have been implicated in the regulation of other muscle-specific genes: a CArG box at -91 bp; myocyte-specific enhancer-binding nuclear factor 1 binding site homologies at -58, -535, and -583 bp; and a muscle-CAAT consensus sequence at -394 bp relative to the cap site. Our observation that only 149 bp of upstream sequence is required for muscle-specific expression of a chimeric CAT gene construct further implicates the CArG and myocyte-specific enhancer-binding nuclear factor 1 binding homologies as important domains in the regulation of this gene. On the other hand, the unique profile of myogenic cell line-specific induction displayed by our DMD promoter-CAT gene constructs suggests that other as yet undefined cis-acting sequences and/or trans-acting factors may also be involved.
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