ArticleJournal of structural biology2013
Maximizing the potential of electron cryomicroscopy data collected using direct detectors.
Article in Journal of structural biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography.Frontiers in molecular biosciences · 2024Review
- Miniaturized Sample Preparation for Transmission Electron Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Cryo-EM in drug discovery: achievements, limitations and prospects.Nature reviews. Drug discovery · 2018Review
- Structure and Immune Recognition of the HIV Glycan Shield.Annual review of biophysics · 2018Article
- Iterative Molecular Dynamics-Rosetta Membrane Protein Structure Refinement Guided by Cryo-EM Densities.Journal of chemical theory and computation · 2017Article
- Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy.Protein science : a publication of the Protein Society · 2017Article
- Methods to account for movement and flexibility in cryo-EM data processing.Methods (San Diego, Calif.) · 2016Article
- Cryo electron microscopy to determine the structure of macromolecular complexes.Methods (San Diego, Calif.) · 2016Review
- Symmetry-mismatch reconstruction of genomes and associated proteins within icosahedral viruses using cryo-EM.Biophysics reports · 2016Article
- Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.Journal of structural biology · 2015Article
- Near-atomic resolution reconstructions using a mid-range electron microscope operated at 200 kV.Journal of structural biology · 2014Article
- High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles.Protoplasma · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Single-particle electron cryomicroscopy is undergoing a technical revolution due to the recent developments of direct detectors. These new recording devices detect electrons directly (i.e. without conversion into light) and feature significantly improved detective quantum efficiencies and readout rates as compared to photographic films or CCDs. We evaluated here the potential of one such detector (Gatan K2 Summit) to enable the achievement of near-atomic resolution reconstructions of biological specimens when coupled to a widely used, mid-range transmission electron microscope (FEI TF20 Twin). Compensating for beam-induced motion and stage drift provided a 4.4Å resolution map of Sulfolobus turreted icosahedral virus (STIV), which we used as a test particle in this study. Several motion correction and dose fractionation procedures were explored and we describe their influence on the resolution of the final reconstruction. We also compared the quality of this data to that collected with a FEI Titan Krios microscope equipped with a Falcon I direct detector, which provides a benchmark for data collected using a high-end electron microscope.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.