ArticleJournal of biomechanics2013
Applied osmotic loading for promoting development of engineered cartilage.
Article in Journal of biomechanics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Skeletal dysplasia-causing mutations in TRPV4 alter the chondrocyte transcriptomic response to mechanical loading.American journal of physiology. Cell physiology · 2025Article
- Toward defining the role of the synovium in mitigating normal articular cartilage wear and tear.Journal of biomechanics · 2023Article
- Intracellular Calcium and Sodium Modulation of Self-Assembled Neocartilage Using Costal Chondrocytes.Tissue engineering. Part A · 2022Article
- Biophysical Modulation of Mesenchymal Stem Cell Differentiation in the Context of Skeletal Repair.International journal of molecular sciences · 2022Review
- Hyperosmolarity benefits cartilage regeneration by enhancing expression of chondrogenic markers and reducing inflammatory markers.In vitro cellular & developmental biology. Animal · 2021Article
- The Role of Chondrocyte Morphology and Volume in Controlling Phenotype-Implications for Osteoarthritis, Cartilage Repair, and Cartilage Engineering.Current rheumatology reports · 2019Review
- Polymeric mesh and insulin-like growth factor 1 delivery enhance cell homing and graft-cartilage integration.Annals of the New York Academy of Sciences · 2019Article
- Effect of biomechanical stress on endogenous antioxidant networks in bovine articular cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Concise Review: Mesenchymal Stem Cells for Functional Cartilage Tissue Engineering: Taking Cues from Chondrocyte-Based Constructs.Stem cells translational medicine · 2017Review
- Mesenchymal Stem Cells for Osteochondral Tissue Engineering.Methods in molecular biology (Clifton, N.J.) · 2016Article
- TRPV4 as a therapeutic target for joint diseases.Naunyn-Schmiedeberg's archives of pharmacology · 2015Review
- Analyzing the effects of mechanical and osmotic loading on glycosaminoglycan synthesis rate in cartilaginous tissues.Journal of biomechanics · 2015Article
- Passage-dependent relationship between mesenchymal stem cell mobilization and chondrogenic potential.Osteoarthritis and cartilage · 2015Article
- Promoting increased mechanical properties of tissue engineered neocartilage via the application of hyperosmolarity and 4α-phorbol 12,13-didecanoate (4αPDD).Journal of biomechanics · 2014Article
- Effect of long-term osmotic loading culture on matrix synthesis from intervertebral disc cells.BioResearch open access · 2014Article
- Biomechanics and mechanobiology in functional tissue engineering.Journal of biomechanics · 2014Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
This study investigated the potential use of static osmotic loading as a cartilage tissue engineering strategy for growing clinically relevant grafts from either synovium-derived stem cells (SDSCs) or chondrocytes. Bovine SDSCs and chondrocytes were individually encapsulated in 2% w/v agarose and divided into chondrogenic media of osmolarities 300 (hypotonic), 330 (isotonic), and 400 (hypertonic, physiologic) mOsM for up to 7 weeks. The application of hypertonic media to constructs comprised of SDSCs or chondrocytes led to increased mechanical properties as compared to hypotonic (300mOsM) or isotonic (330mOsM) media (p<0.05). Constant exposure of SDSC-seeded constructs to 400mOsM media from day 0 to day 49 yielded a Young's modulus of 513±89kPa and GAG content of 7.39±0.52%ww on day 49, well within the range of values of native, immature bovine cartilage. Primary chondrocyte-seeded constructs achieved almost as high a Young's modulus, reaching 487±187kPa and 6.77±0.54%ww (GAG) for the 400mOsM condition (day 42). These findings suggest hypertonic loading as a straightforward strategy for 3D cultivation with significant benefits for cartilage tissue engineering strategies. In an effort to understand potential mechanisms responsible for the observed response, cell volume measurements in response to varying osmotic conditions were evaluated in relation to the Boyle-van't Hoff (BVH) law. Results confirmed that chondrocytes behave as perfect osmometers; however SDSCs deviated from the BVH relation.
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Registered trials
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