ArticleBiomaterials2013
Aqueous two-phase printing of cell-containing contractile collagen microgels.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 61 citations in OpenAlex.
- SpheroScan: A User-Friendly Deep Learning Tool for Spheroid Image Analysis.bioRxiv : the preprint server for biology · 2023Article
- Triple-negative breast cancer cells invade adipocyte/preadipocyte-encapsulating geometrically inverted mammary organoids.Integrative biology : quantitative biosciences from nano to macro · 2023Article
- Article
- Enhanced Bone Remodeling After Fracture Priming.Calcified tissue international · 2022Article
- The case for cancer-associated fibroblasts: essential elements in cancer drug discovery?Future drug discovery · 2022Review
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
- Aqueous two-phase deposition and fibrinolysis of fibroblast-laden fibrin micro-scaffolds.Biofabrication · 2021Article
- A tough act to follow: collagen hydrogel modifications to improve mechanical and growth factor loading capabilities.Materials today. Bio · 2021Review
- Cell-Inspired All-Aqueous Microfluidics: From Intracellular Liquid-Liquid Phase Separation toward Advanced Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Kinetic Analysis of Label-Free Microscale Collagen Gel Contraction Using Machine Learning-Aided Image Analysis.Frontiers in bioengineering and biotechnology · 2020Article
- Biofabrication of phenotypic pulmonary fibrosis assays.Biofabrication · 2019Review
- Investigating Fibroblast-Induced Collagen Gel Contraction Using a Dynamic Microscale Platform.Frontiers in bioengineering and biotechnology · 2019Article
- Collagen Partition in Polymeric Aqueous Two-Phase Systems for Tissue Engineering.Frontiers in chemistry · 2018Article
- Controlled Tubular Unit Formation from Collagen Film for Modular Tissue Engineering.ACS biomaterials science & engineering · 2017Article
- Cell-laden microfluidic microgels for tissue regeneration.Lab on a chip · 2016Review
- Microscale 3D collagen cell culture assays in conventional flat-bottom 384-well plates.Journal of laboratory automation · 2015Article
- Spatial regulation of controlled bioactive factor delivery for bone tissue engineering.Advanced drug delivery reviews · 2015Review
- Supersoft lithography: candy-based fabrication of soft silicone microstructures.Lab on a chip · 2015Article
- High Throughput, Polymeric Aqueous Two-Phase Printing of Tumor Spheroids.Advanced functional materials · 2014Article
- Defined topologically-complex protein matrices to manipulate cell shape via three-dimensional fiber-like patterns.Lab on a chip · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
This work describes the use of aqueous two-phase systems to print cell-containing contractile collagen microdroplets. The fully aqueous conditions enable convenient formation of sub-microliter 'microgels' that are much smaller than otherwise possible to fabricate while maintaining high cell viability. The produced microgels contract over several days, mimicking the behavior of macroscale contraction assays, which have been valued as an important biological readout for over three decades. Use of microgels not only reduces reagent consumption and increases throughput of the assay, but also improves transport of molecules into and out of the collagen matrix, thereby enabling efficient and more precise studies of timed stimulation profiles. Utility of the technology is demonstrated by analyzing the effects of TGF-β1 on gel contraction, and we demonstrate that brief 'burst' stimulation profiles in microgels prompt contraction of the matrix, a feature not observed in the conventional macroscale assay. The fully aqueous process also enables the integration of contractile collagen microgels within existing cell culture systems, and we demonstrate proof-of-principle experiments in which a contractile collagen droplet is fabricated in situ on an existing epithelial monolayer. The simplicity, versatility and ability to robustly produce collagen microgels should allow effective translation of this microengineering technology into a variety of research environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.