ArticleBiomaterials2013
In vivo ultrasound visualization of non-occlusive blood clots with thrombin-sensitive contrast agents.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Acoustic Bubble Sensing Techniques and Bioapplications.Biosensors · 2026Review
- Aptamers as a New Frontier in Molecular Cancer Imaging Technologies.Chemical & biomedical imaging · 2025Review
- Development and Characterization of Hemoglobin Microbubbles for Acoustic Blood Oxygen Level Dependent Imaging.ACS sensors · 2024Article
- Aptamer-Functionalized Microbubbles Targeted to P-selectin for Ultrasound Molecular Imaging of Murine Bowel Inflammation.Molecular imaging and biology · 2023Article
- Rapid molecular imaging of active thrombiMolecular therapy. Nucleic acids · 2023Article
- An in situ inferior vena cava ligation-stenosis model to study thrombin generation rates with flow.Thrombosis journal · 2022Article
- Molecular Ultrasound Imaging.Nanomaterials (Basel, Switzerland) · 2020Review
- Design and Application of Stimulus-Responsive Droplets and Bubbles Stabilized by Phospholipid Monolayers.Current opinion in colloid & interface science · 2019Article
- Ultrasound molecular imaging as a non-invasive companion diagnostic for netrin-1 interference therapy in breast cancer.Theranostics · 2018Article
- Thrombin-Activatable Microbubbles as Potential Ultrasound Contrast Agents for the Detection of Acute Thrombosis.ACS applied materials & interfaces · 2017Article
- Nanoparticles Formed by Acoustic Destruction of Microbubbles and Their Utilization for Imaging and Effects on Therapy by High Intensity Focused Ultrasound.Theranostics · 2017Article
- Selective Vaporization of Superheated Nanodroplets for Rapid, Sensitive, Acoustic Biosensing.Advanced healthcare materials · 2015Article
- Applications of aptamers in targeted imaging: state of the art.Current topics in medicinal chemistry · 2015Review
- On-demand droplet fusion: a strategy for stimulus-responsive biosensing in solution.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Smart materials based on DNA aptamers: taking aptasensing to the next level.Sensors (Basel, Switzerland) · 2014Review
- Stimulus-responsive ultrasound contrast agents for clinical imaging: motivations, demonstrations, and future directions.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The use of microbubbles as ultrasound contrast agents is one of the primary methods to diagnose deep venous thrombosis. However, current microbubble imaging strategies require either a clot sufficiently large to produce a circulation filling defect or a clot with sufficient vascularization to allow for targeted accumulation of contrast agents. Previously, we reported the design of a microbubble formulation that modulated its ability to generate ultrasound contrast from interaction with thrombin through incorporation of aptamer-containing DNA crosslinks in the encapsulating shell, enabling the measurement of a local chemical environment by changes in acoustic activity. However, this contrast agent lacked sufficient stability and lifetime in blood to be used as a diagnostic tool. Here we describe a PEG-stabilized, thrombin-activated microbubble (PSTA-MB) with sufficient stability to be used in vivo in circulation with no change in biomarker sensitivity. In the presence of actively clotting blood, PSTA-MBs showed a 5-fold increase in acoustic activity. Specificity for the presence of thrombin and stability under constant shear flow were demonstrated in a home-built in vitro model. Finally, PSTA-MBs were able to detect the presence of an active clot within the vena cava of a rabbit sufficiently small as to not be visible by current non-specific contrast agents. By activating in non-occlusive environments, these contrast agents will be able to detect clots not diagnosable by current contrast agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.