ArticleFrontiers in plant science2013
RuBisCO depletion improved proteome coverage of cold responsive S-nitrosylated targets in Brassica juncea.
Article in Frontiers in plant science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 79 citations in OpenAlex.
- Nitrate reductase-mediated production of nitric oxide enhances cold tolerance in rice seedlings.Scientific reports · 2026Article
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- Toward the commercialization of recombinant pharmaceuticals expressed in plants.Plant biotechnology (Tokyo, Japan) · 2025Article
- Nitric oxide (NO) modulates low temperature-stress signaling via S-nitrosation, a NO PTM, inducing ethylene biosynthesis inhibition leading to enhanced post-harvest shelf-life of agricultural produce.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- The role of nitric oxide and hydrogen sulfide in regulation of redox homeostasis at extreme temperatures in plants.Frontiers in plant science · 2023Review
- Article
- What signals the glyoxalase pathway in plants?Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- MicroRNA Omics Analysis ofBiomolecules · 2021Article
- Plant Proteoforms Under Environmental Stress: Functional Proteins Arising From a Single Gene.Frontiers in plant science · 2021Review
- Comparative Physiological Analysis Reveals the Role of NR-Derived Nitric Oxide in the Cold Tolerance of Forage Legumes.International journal of molecular sciences · 2019Article
- Nitric oxide alleviates cell death through protein S-nitrosylation and transcriptional regulation during the ageing of elm seeds.Journal of experimental botany · 2018Article
- Antioxidant Systems are Regulated by Nitric Oxide-Mediated Post-translational Modifications (NO-PTMs).Frontiers in plant science · 2016Review
- Circadian Profiling of the Arabidopsis Proteome Using 2D-DIGE.Frontiers in plant science · 2016Article
- Combined Cytological and Transcriptomic Analysis Reveals a Nitric Oxide Signaling Pathway Involved in Cold-Inhibited Camellia sinensis Pollen Tube Growth.Frontiers in plant science · 2016Article
- Nitric Oxide Level Is Self-Regulating and Also Regulates Its ROS Partners.Frontiers in plant science · 2016Article
- Toward the complete proteome of Synechocystis sp. PCC 6803.Photosynthesis research · 2015Review
- Differential inhibition of Arabidopsis superoxide dismutases by peroxynitrite-mediated tyrosine nitration.Journal of experimental botany · 2015Article
- Nitric oxide triggers a concentration-dependent differential modulation of superoxide dismutase (FeSOD and Cu/ZnSOD) activity in sunflower seedling roots and cotyledons as an early and long distance signaling response to NaCl stress.Plant signaling & behavior · 2015Article
- Time to dig deep into the plant proteome: a hunt for low-abundance proteins.Frontiers in plant science · 2015Article
- Nitric oxide, antioxidants and prooxidants in plant defence responses.Frontiers in plant science · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although in the last few years good number of S-nitrosylated proteins are identified but information on endogenous targets is still limiting. Therefore, an attempt is made to decipher NO signaling in cold treated Brassica juncea seedlings. Treatment of seedlings with substrate, cofactor and inhibitor of Nitric-oxide synthase and nitrate reductase (NR), indicated NR mediated NO biosynthesis in cold. Analysis of the in vivo thiols showed depletion of low molecular weight thiols and enhancement of available protein thiols, suggesting redox changes. To have a detailed view, S-nitrosylation analysis was done using biotin switch technique (BST) and avidin-affinity chromatography. Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is S-nitrosylated and therefore, is identified as target repeatedly due to its abundance. It also competes out low abundant proteins which are important NO signaling components. Therefore, RuBisCO was removed (over 80%) using immunoaffinity purification. Purified S-nitrosylated RuBisCO depleted proteins were resolved on 2-D gel as 110 spots, including 13 new, which were absent in the crude S-nitrosoproteome. These were identified by nLC-MS/MS as thioredoxin, fructose biphosphate aldolase class I, myrosinase, salt responsive proteins, peptidyl-prolyl cis-trans isomerase and malate dehydrogenase. Cold showed differential S-nitrosylation of 15 spots, enhanced superoxide dismutase activity (via S-nitrosylation) and promoted the detoxification of superoxide radicals. Increased S-nitrosylation of glyceraldehyde-3-phosphate dehydrogenase sedoheptulose-biphosphatase, and fructose biphosphate aldolase, indicated regulation of Calvin cycle by S-nitrosylation. The results showed that RuBisCO depletion improved proteome coverage and provided clues for NO signaling in cold.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.