ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2012
Comparison of different protocols for the extraction of microbial DNA from reef corals.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- A Comparison of Different Protocols for the Extraction of Microbial DNA Inhabiting Synthetic Mars Simulant Soil.Microorganisms · 2024Article
- Inter-comparison of marine microbiome sampling protocols.ISME communications · 2023Article
- Metabolically Active Prokaryotes and Actively Transcribed Antibiotic Resistance Genes in Sewer Systems: Implications for Public Health and Microbially Induced Corrosion.Microbial ecology · 2022Article
- Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics.Frontiers in microbiology · 2022Review
- Evaluation of direct and biochar carrier-based inoculation of Bacillus sp. on As- and Pb-contaminated technosol: effect on metal(loid) availability, Salix viminalis growth, and soil microbial diversity/activity.Environmental science and pollution research international · 2021Article
- Toxicity of phthalate esters to lettuce (Lactuca sativa) and the soil microbial community under different soil conditions.PloS one · 2018Article
- Article
- The microbial biosphere of the coralPeerJ · 2017Article
- Impact of oil spills on coral reefs can be reduced by bioremediation using probiotic microbiota.Scientific reports · 2015Article
- A novel marine mesocosm facility to study global warming, water quality, and ocean acidification.Ecology and evolution · 2015Article
- Climate change affects key nitrogen-fixing bacterial populations on coral reefs.The ISME journal · 2014Article
- Comparison of DNA extraction protocols for microbial communities from soil treated with biochar.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
Corrections and comments
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Authors and funding
28 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to test different protocols for the extraction of microbial DNA from the coral Mussismilia harttii. Four different commercial kits were tested, three of them based on methods for DNA extraction from soil (FastDNA SPIN Kit for soil, MP Bio, PowerSoil DNA Isolation Kit, MoBio, and ZR Soil Microbe DNA Kit, Zymo Research) and one kit for DNA extraction from plants (UltraClean Plant DNA Isolation Kit, MoBio). Five polyps of the same colony of M. harttii were macerated and aliquots were submitted to DNA extraction by the different kits. After extraction, the DNA was quantified and PCR-DGGE was used to study the molecular fingerprint of Bacteria and Eukarya. Among the four kits tested, the ZR Soil Microbe DNA Kit was the most efficient with respect to the amount of DNA extracted, yielding about three times more DNA than the other kits. Also, we observed a higher number and intensities of DGGE bands for both Bacteria and Eukarya with the same kit. Considering these results, we suggested that the ZR Soil Microbe DNA Kit is the best adapted for the study of the microbial communities of corals.
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Registered trials
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