ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2012
Optimization of exopolysaccharides production from a novel strain of Ganoderma lucidum CAU5501 in submerged culture.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 47 citations in OpenAlex.
- Exploring Ganoderma lucidum: morphology, cultivation and market potential.World journal of microbiology & biotechnology · 2024Review
- Production of Ganodiesel from the biomass ofHeliyon · 2024Article
- Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization.Applied biochemistry and biotechnology · 2021Article
- Article
- Differences in Production, Composition, and Antioxidant Activities of Exopolymeric Substances (EPS) Obtained from Cultures of EndophyticMolecules (Basel, Switzerland) · 2020Article
- Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identifiedAIMS microbiology · 2019Article
- Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture ofBiochimie open · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
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Abstract
This study aimed at optimizing the medium of a new Ganoderma lucidum strain CAU5501 to enhance the yield of exopolysaccharides (EPS) and mycelial growth. Firstly, the suitable level of glucose, magnesium, phosphate and C/N ratio was determined by single factor experiment. Subsequently, the optimum concentrations of these medium components were investigated using the orthogonal matrix method. The results indicated that the higher levels of EPS were correlated with the level of cell growth when glucose concentration was studied (data no show). The optimum medium for EPS yield was found to be 70 g/l glucose, 5 C/N ratio, 2.5 g/l KH2PO4, 0.75 g/l MgSO4·7H2O, and for mycelial growth was 50 g/l glucose, 5 C/N ratio, 1.5 g/l KH2PO4, 0.5 g/l MgSO4·7H2O. When cultivated in the obtained optimal media in 3 L shake flask, compared to the basal medium, the EPS yield increased markedly from 1.003 to 1.723 g/l, and the mycelium formation was also markedly improved from 2.028 to 7.235 g/l. Results obtained in this study are beneficial to further study for enhancing the production of Ganoderma lucidum polysaccharides in large scale commercialized production.
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