ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2011
Characterization of a chitinase with antifungal activity from a native Serratia marcescens B4A.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 78 citations in OpenAlex.
- A Chitinase Gene Belonging to Serratia marcescens GBS19 Reveals Horizontal Gene Transfer within Bacterial Strains Besides its Biocontrol Potential Against Myzus persicae.Biochemical genetics · 2026Article
- Control of anthracnose caused by Colletotrichum truncatum on dragon fruit (Hylocereus undatus) using recombinant chitinase.Archives of microbiology · 2026Article
- Production, optimization, characterization, and application of chitinase from Bacillus proteolyticus isolated from the rhizosphere of diseased arecanut palm.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Functional and Genomic Characterization ofPlants (Basel, Switzerland) · 2026Article
- From Nature to Nanotech: Customizing Essential Oil Delivery for a Reduced Fungicide in Agriculture.ACS omega · 2026Review
- The impact of planting times and cultivars on the excitation of innate-immunity response against populations and severity of plant parasitic nematodes in faba bean (Vicia faba L.) field.BMC plant biology · 2025Article
- The Functional Characterization of an AA10 Lytic Polysaccharide Monooxygenase fromFoods (Basel, Switzerland) · 2025Article
- Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- The acidic latex protein fromPeerJ · 2025Article
- Chitinase-functionalized UiO-66 framework nanoparticles active against multidrug-resistant Candida Auris.BMC microbiology · 2024Article
- Biocontrol potential of endophytic fungi against phytopathogenic nematodes on potato (Solanum tuberosum L.).Scientific reports · 2024Article
- Invisible Inhabitants of Plants and a Sustainable Planet: Diversity of Bacterial Endophytes and their Potential in Sustainable Agriculture.Indian journal of microbiology · 2024Review
- Functional Comparison of Three Chitinases from Symbiotic Bacteria of Entomopathogenic Nematodes.Toxins · 2024Article
- Phloretin Inhibits Quorum Sensing and Biofilm Formation inMolecules (Basel, Switzerland) · 2023Article
- An overview of viral chitinases: General properties and biotechnological potential.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Fish Skin Mucus Extracts: An Underexplored Source of Antimicrobial Agents.Marine drugs · 2023Review
- Comparison of in vitro Antifungal Activity Methods Using Extract of Chitinase-producing Aeromonas sp. BHC02.The protein journal · 2023Article
- Microbial chitinases and their relevance in various industries.Folia microbiologica · 2023Review
- Genetic Determinants of Antagonistic Interactions and the Response of New Endophytic StrainInternational journal of molecular sciences · 2022Article
- Zooplankton act as cruise ships promoting the survival and pathogenicity of pathogenic bacteria.Microbiology and immunology · 2022Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45°C. Enzyme was stable in 55°C for 20 min and at a pH range of 3-9 for 90 min at 25°C. When the temperature was raised to 60°C, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to stimulate the chitinase activity. In addition, Iodoacetamide and Idoacetic acid did not inhibit enzyme activity, indicating that cysteine residues are not part of the catalytic site of chitinase. Finally, chitinase activity was further monitored by scanning electronic microscopy data in which progressive changes in chitin porosity appeared upon treatment with chitinase. This enzyme exhibited antifungal activity against Rhizoctonia solani, Bipolaris sp, Alternaria raphani, Alternaria brassicicola, revealing a potential application for the industry with potentially exploitable significance. Fungal chitin shows some special features, in particular with respect to chemical structure. Difference in chitinolytic ability must result from the subsite structure in the enzyme binding cleft. This implies that why the enzyme didn't have significant antifungal activity against other Fungi.
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