ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2011
Comparative analysis of cultural isolation and PCR based assay for detection of campylobacter jejuni in food and faecal samples.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- The effect of changing diagnostic method from culture to PCR on the number of episodes of human campylobacteriosis in Denmark: a retrospective study (2015-2022).Microbiology spectrum · 2024Article
- An Integrated Paper Microfluidic Device Based on Isothermal Amplification for Simple Sample-to-Answer Detection ofApplied and environmental microbiology · 2023Article
- First Report ofAnimals : an open access journal from MDPI · 2020Article
- Prevalence, molecular detection, and virulence gene profiles ofVeterinary world · 2020Article
- A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli.Journal of nanobiotechnology · 2019Article
- Prevalence and Antibiotic Resistance against Tetracycline inFrontiers in microbiology · 2017Article
- Prevalence ofIranian journal of veterinary research · 2017Article
- Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and anti-bio gram.Veterinary world · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8, respectively, could be detected by isolation whereas 6, 3 and 10, respectively, were found positive by PCR. However, among food samples viz. beef (30), milk (35), cheese (30), only one beef sample was detected both by culture as well as PCR. Additionally, PCR was found to be more sensitive for C. jejuni detection in spiked faecal and food samples (96.1% each) as relative to culture isolation which could detect the organism in 86.7% and 80% samples, respectively. The results depicted the superior efficacy of PCR for rapid screening of samples owing to its high sensitivity, specificity and automation potential.
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