Evidence map›Paper›PMID 24031494›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2010

A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis.

Gavish Kumar, Hari Shankar, Deepa Bisht, Prashant Sharma, Neelja Singhal, V M Katoch, Beenu Joshi

Open access · hybridAbstract read
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Gavish KumarDepartment of Immunology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR) , Tajganj , Agra 282 001(India).
Hari Shankar
Deepa Bisht
Prashant Sharma
Neelja Singhal
V M Katoch
Beenu Joshi
National JALMA Institute for Leprosy & Other Mycobacterial Diseases · INIndian Council of Medical Research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.

Indexed as

Culture filtrate proteinsM. tuberculosisSDS-TCA procedureTwo-dimensional gel electrophoresis

Identifiers

PMID24031494
PMCPMC3768669
OpenAlexW2101209641

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.