ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2009
A RT-PCR assay for the detection of encephalomycarditis virus infections in pigs.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Litters of Various-Sized Mummies (LVSM) and Stillborns after Porcine Reproductive and Respiratory Syndrome Virus Type 1 Infection-A Case Report.Veterinary sciences · 2023Article
- A Validated Multiplex Real-Time PCR Assay for the Diagnosis of InfectiousFrontiers in microbiology · 2020Article
- Evaluation of a duplex reverse-transcription real-time PCR assay for the detection of encephalomyocarditis virus.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2018Article
- Multi-Target Strategy for Pan/Foot-and-Mouth Disease Virus (FMDV) Detection: A Combination of Sequences Analysis,Frontiers in veterinary science · 2018Article
- Clinical problems due to encephalomyocarditis virus infections in two pig herds.Porcine health management · 2016Article
- Inhibition of type I interferon-mediated antiviral action in human glioma cells by the IKK inhibitors BMS-345541 and TPCA-1.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Encephalomyocarditis virus (EMCV) infections can cause losses in pig farms all over the world. Rapid, sensitive and unequivocal detection of this virus is therefore essential for the diagnosis and control of the disease. An RT-PCR assay was developed, optimized and evaluated for encephalomyocarditis virus detection in organ based on a pair of primers that amplifies a 165 bp DNA fragment from a highly conserved nucleotide region of the viral 3D glycoprotein. PCR products of the expected size were obtained from Cuban EMCV 744/03 strain. Non-specific reactions were not observed when other porcine RNA genome viruses and uninfected cells were used. The analytical sensitivity of the test was estimated to be 2 TCID50/50 μL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
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