ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2009
Characterization of recombinant MSP5 Anaplasma marginale Havana isolate.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- A Cross-Sectional Serological Study to Assess the Prevalence and Risk Factors of Anaplasmosis in Dromedary Camels in Punjab, Pakistan.Veterinary sciences · 2024Article
- Molecular detection and characterization of Anaplasma marginale and Babesia canis vogeli infecting dogs in Luxor, Egypt.Scientific reports · 2024Article
- Molecular detection and characterization ofFrontiers in veterinary science · 2023Article
- Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states.BMC veterinary research · 2020Article
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4 authors.
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Abstract
Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the genus Anaplasma and in all isolates of A. marginale. The aim of the present work was to carry out the cloning, sequencing and characterization of the recombinant MSP5 Anaplasma marginale Havana isolate. The sequence of the msp5 gene of Anaplasma marginale Havana isolate with a size of 633 pb was determined (Acc. No. AY527217). This gene was cloned into pRSETB vector and expressed in Escherichia coli. The MSP5 protein was recognized by the monoclonal antibody ANAF16C1 and it showed a high similitude percent with the gene sequence described for other Anaplasma marginale isolates. These data are very important for the development of a diagnostic test for A. marginale using the MSP5 recombinant protein.
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