ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2009
Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Lipase Production by Pseudomonas aeruginosa ATCC 9027: Process Optimization, Kinetic Modeling and Cost Assessment.Current microbiology · 2026Article
- Catalytically active inclusion bodies of cold-adapted lipase: production and its industrial potential.AMB Express · 2025Article
- Immobilized inclusion bodies of recombinant cold-adaptive lipase from Antarctic Pseudomonas sp. as catalysts.Applied microbiology and biotechnology · 2025Article
- Linking the Metabolic Activity of Plastic-Degrading Fungi to Their Taxonomy and Evolution.Journal of fungi (Basel, Switzerland) · 2025Review
- Production and characterization of a promising microbial-derived lipase enzyme targeting BCL-2 gene expression in hepatocellular carcinoma.Microbial cell factories · 2025Article
- Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa.Molecular biology reports · 2024Article
- The main Aflatoxin B1 degrading enzyme inHeliyon · 2022Article
- Alkaline lipase production by novel meso-tolerant psychrophilic Exiguobacterium sp. strain (AMBL-20) isolated from glacier of northeastern Pakistan.Archives of microbiology · 2021Article
- Effect of mutation at oxyanion hole residu (H110F) on activity of Lk4 lipase.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- A cleaner process of deinking waste paper pulp using Pseudomonas mendocina ED9 lipase supplemented enzyme cocktail.Environmental science and pollution research international · 2020Article
- A hydrolase with esterase activity expressed from a fosmid gene bank prepared from DNA of a North West Himalayan glacier frozen soil sample.3 Biotech · 2019Article
- Cold-active extracellular lipase: Expression in Sf9 insect cells, purification, and catalysis.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial StrainMolecules (Basel, Switzerland) · 2019Article
- Production and purification of an alkaline lipase from3 Biotech · 2018Article
- Characterization of ML-005, a Novel Metaproteomics-Derived Esterase.Frontiers in microbiology · 2018Article
- Purification and characterization of lipase byJournal, genetic engineering & biotechnology · 2017Article
- Identification of lipolytic enzymes isolated from bacteria indigenous toBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.FEBS open bio · 2016Article
- Kinetic modeling, production and characterization of an acidic lipase produced by Enterococcus durans NCIM5427 from fish waste.Journal of food science and technology · 2015Article
- An oxidant and organic solvent tolerant alkaline lipase by P. aeruginosa mutant: downstream processing and biochemical characterization.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An extra cellular lipase was isolated and purified from the culture broth of Pseudomonas aeruginosa SRT 9 to apparent homogeneity using ammonium sulfate precipitation followed by chromatographic techniques on phenyl Sepharose CL- 4B and Mono Q HR 5/5 column, resulting in a purification factor of 98 fold with specific activity of 12307.8 U/mg. The molecular weight of the purified lipase was estimated by SDS-PAGE to be 29 kDa with isoelectric point of 4.5. Maximum lipase activity was observed in a wide range of temperature and pH values with optimum temperature of 55ºC and pH 6.9. The lipase preferably acted on triacylglycerols of long chain (C14-C16) fatty acids. The lipase was inhibited strongly by EDTA suggesting the enzyme might be metalloprotein. SDS and metal ions such as Hg(2+), Zn(2+), Cu(2+), Ag(2+) and Fe(2+) decreased the lipase activity remarkedly. Its marked stability and activity in organic solvents suggest that this lipase is highly suitable as a biotechnological tool with a variety of applications including organo synthetic reactions and preparation of enantiomerically pure pharmaceuticals. The Km and Vmax value of the purified enzyme for triolein hydrolysis were calculated to be 1.11 mmol/L and 0.05 mmol/L/min respectively.
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