ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2014
Discrimination of Aspergillus lentulus from Aspergillus fumigatus by Raman spectroscopy and MALDI-TOF MS.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Comparison of Morphological, Virulence, and Antifungal Susceptibility Characteristics WithinInfection and drug resistance · 2025Article
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- Use of MALDI-TOF MS to Discriminate between Aflatoxin B1-Producing and Non-Producing Strains ofMolecules (Basel, Switzerland) · 2022Article
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- The robust and rapid role of molecular testing in precision fungal diagnostics: A case report.Medical mycology case reports · 2020Article
- Rapid and label-free detection of COVID-19 using coherent anti-Stokes Raman scattering microscopy.MRS communications · 2020Article
- Clinical and Microbiological Characterization of Invasive Pulmonary Aspergillosis Caused byFrontiers in microbiology · 2020Article
- Antibiotic Resistance and Molecular Epidemiological Characteristics ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Fusion of MALDI Spectrometric Imaging and Raman Spectroscopic Data for the Analysis of Biological Samples.Frontiers in chemistry · 2018Article
- Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species.BMC microbiology · 2017Article
- Decision criteria for MALDI-TOF MS-based identification of filamentous fungi using commercial and in-house reference databases.BMC microbiology · 2017Article
- Conventional Morphology Versus PCR Sequencing, rep-PCR, and MALDI-TOF-MS for Identification of Clinical Aspergillus Isolates Collected Over a 2-Year Period in a University Hospital at Kayseri, Turkey.Journal of clinical laboratory analysis · 2016Article
- Evaluation of the Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry System for Identification of Clinically Relevant Filamentous Fungi.Journal of clinical microbiology · 2016Article
- Aspergillus fumigatus-Related Species in Clinical Practice.Frontiers in microbiology · 2016Review
- Early invasive pulmonary aspergillosis in a kidney transplant recipient caused by Aspergillus lentulus: first Brazilian report.Mycopathologia · 2015Article
- Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section Fumigati.Frontiers in microbiology · 2015Review
- Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole.BMC microbiology · 2014Article
- Identification and nomenclature of the genus Penicillium.Studies in mycology · 2014Article
- Phylogeny, identification and nomenclature of the genus Aspergillus.Studies in mycology · 2014Article
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9 authors.
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Abstract
In 2005, a new sibling species of Aspergillus fumigatus was discovered: Aspergillus lentulus. Both species can cause invasive fungal disease in immune-compromised patients. The species are morphologically very similar. Current techniques for identification are PCR-based or morphology-based. These techniques are labour-intense and not sufficiently discriminatory. Since A. lentulus is less susceptible to several antifungal agents, it is important to correctly identify the causative infectious agent in order to optimize antifungal therapy. In this study we determined whether Raman spectroscopy and/or MALDI-TOF MS were able to differentiate between A. lentulus and A. fumigatus. For 16 isolates of A. lentulus and 16 isolates of A. fumigatus, Raman spectra and peptide profiles were obtained using the Spectracell and MALDI-TOF MS (VITEK MS RUO, bioMérieux) respectively. In order to obtain reliable Raman spectra for A. fumigatus and A. lentulus, the culture medium needed to be adjusted to obtain colourless conidia. Only Raman spectra obtained from colourless conidia were reproducible and correctly identified 25 out of 32 (78 %) of the Aspergillus strains. For VITEK MS RUO, no medium adjustments were necessary. Pigmented conidia resulted in reproducible peptide profiles as well in this case. VITEK MS RUO correctly identified 100 % of the Aspergillus isolates, within a timeframe of approximately 54 h including culture. Of the two techniques studied here, VITEK MS RUO was superior to Raman spectroscopy in the discrimination of A. lentulus from A. fumigatus. VITEK MS RUO seems to be a successful technique in the daily identification of Aspergillus spp. within a limited timeframe.
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