ArticleWorld journal of microbiology & biotechnology2014
Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate.
Article in World journal of microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 27 citations in OpenAlex.
- Functional Characterization and Promoter Analysis of a MeJA-Responsive Squalene Synthase GeneInternational journal of molecular sciences · 2026Article
- Multi-omics-guided discovery of CYP5035 enables heterologous production and engineering of pachymic acid in yeast.Journal of biological engineering · 2026Article
- Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing.Microbial cell factories · 2024Article
- New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal SpeciesJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Genome editing in the edible fungusFrontiers in microbiology · 2022Article
- Positive selection and functional divergence of farnesyl pyrophosphate synthase genes in plants.BMC molecular biology · 2017Article
- Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling).Chinese medicine · 2016Article
- Cloning and characterization of farnesyl diphosphate synthase gene involved in triterpenoids biosynthesis from Poria cocos.International journal of molecular sciences · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Squalene synthase (SQS) catalyzes the condensation of two molecules of farnesyl diphosphate to give presqualene diphosphate and the subsequent rearrangement to form squalene. The gene encoding squalene synthase was cloned from Poria cocos by degenerate PCR and inverse PCR. The open reading frame of the gene is 1,497 bp, which encodes 499 amino acid residues. A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. The treatment of P. cocos with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of P. cocos sqs gene and the content of squalene in P. cocos. The transcriptional level of sqs gene was approximately fourfold higher than the control sample and the squalene content reached 128.62 μg/g, when the concentration of MeJA was 300 μM after 72 h induction.
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