ArticleGenes & development2013
Regulation of pluripotency in male germline stem cells by Dmrt1.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- A Multi-omics Atlas of Gonadal Sex Differences and Characterization of the dmrt1 Gene in Gymnocypris Eckloni Herzenstein.Marine biotechnology (New York, N.Y.) · 2026Article
- SMAD3 and HIF-1α orchestrate metabolic transition to aerobic glycolysis as a critical prerequisite for spontaneous reprogramming of spermatogonial stem cells.Stem cell research & therapy · 2025Article
- Article
- From unipotency to pluripotency: deciphering protein networks and signaling pathways in the generation of embryonic stem-like cells from murine spermatogonial stem cells.BMC genomics · 2025Article
- Unraveling the Significance of Nanog in the Generation of Embryonic Stem-like Cells from Spermatogonia Stem Cells: A Combined In Silico Analysis and In Vitro Experimental Approach.International journal of molecular sciences · 2024Article
- A rapid and stable spontaneous reprogramming system of Spermatogonial stem cells to Pluripotent State.Cell & bioscience · 2023Article
- The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting.Protein & cell · 2023Article
- Transcription factorZoological research · 2023Article
- Characterization of the In Vitro Cultured Ovarian Cells in the Asian Yellow Pond Turtle (Biology · 2022Article
- Application of CRISPR/Cas Technology in Spermatogenesis Research and Male Infertility Treatment.Genes · 2022Review
- Histone methyltransferase DOT1L is essential for self-renewal of germline stem cells.Genes & development · 2022Article
- Analysis of chromatin accessibility in p53 deficient spermatogonial stem cells for high frequency transformation into pluripotent state.Cell proliferation · 2022Article
- Genomic stability of mouse spermatogonial stem cells in vitro.Scientific reports · 2021Article
- Increased de novo DNA Methylation Enzymes in Sperm of Individuals with Varicocele.Cell journal · 2021Article
- DMRT1 gene disruption alone induces incomplete gonad feminization in chicken.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency.Nature communications · 2021Article
- Epigenetic Regulation of Spermatogonial Stem Cell Homeostasis: From DNA Methylation to Histone Modification.Stem cell reviews and reports · 2021Review
- Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells.Zoological research · 2021Article
- mRBPome capture identifies the RNA-binding protein TRIM71, an essential regulator of spermatogonial differentiation.Development (Cambridge, England) · 2020Article
- PACT/PRKRA and p53 regulate transcriptional activity of DMRT1.Genetics and molecular biology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermatogonial stem cells (SSCs) present the potential to acquire pluripotency under specific culture conditions. However, the frequency of pluripotent cell derivation is low, and the mechanism of SSC reprogramming remains unknown. In this study, we report that induction of global DNA hypomethylation in germline stem (GS) cells (cultured SSCs) induces pluripotent cell derivation. When DNA demethylation was triggered by Dnmt1 depletion, GS cells underwent apoptosis. However, GS cells were converted into embryonic stem (ES)-like cells by double knockdown of Dnmt1 and p53. This treatment down-regulated Dmrt1, a gene involved in sexual differentiation, meiosis, and pluripotency. Dmrt1 depletion caused apoptosis of GS cells, but a combination of Dmrt1 and p53 depletion also induced pluripotency. Functional screening of putative Dmrt1 target genes revealed that Dmrt1 depletion up-regulates Sox2. Sox2 transfection up-regulated Oct4 and produced pluripotent cells. This conversion was enhanced by Oct1 depletion, suggesting that the balance of Oct proteins maintains SSC identity. These results suggest that spontaneous SSC reprogramming is caused by unstable DNA methylation and that a Dmrt1-Sox2 cascade is critical for regulating pluripotency in SSCs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.