ArticleScientific reports2013
Quantitative full time course analysis of nonlinear enzyme cycling kinetics.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 51 citations in OpenAlex.
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- Enzyme activation by urea reveals the interplay between conformational dynamics and substrate binding: a single-molecule FRET study.bioRxiv : the preprint server for biology · 2024Article
- Optimizing Trilobatin Production via Screening and Modification of Glycosyltransferases.Molecules (Basel, Switzerland) · 2024Article
- Effects of Phosphorylation on the Activity, Inhibition and Stability of Carbonic Anhydrases.International journal of molecular sciences · 2023Article
- High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors.3 Biotech · 2021Article
- An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA.Life (Basel, Switzerland) · 2021Article
- Measuring the Complex Effects of the Single-Stranded DNA-Binding Protein gp2.5 on Primer Synthesis and Extension by the Bacteriophage T7 Replisome.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Structural and functional insights into Archaeoglobus fulgidus m2G10 tRNA methyltransferase Trm11 and its Trm112 activator.Nucleic acids research · 2020Article
- GTP hydrolysis by Synechocystis IM30 does not decisively affect its membrane remodeling activity.Scientific reports · 2020Article
- Numerical Analysis of Time-Dependent Inhibition by MDMA.Drug metabolism and disposition: the biological fate of chemicals · 2020Article
- Beyond the Michaelis-Menten equation: Accurate and efficient estimation of enzyme kinetic parameters.Scientific reports · 2017Article
- Biophysical modeling of in vitro and in vivo processes underlying regulated photoprotective mechanism in cyanobacteria.Photosynthesis research · 2017Article
- The anti-inflammatory compound palmitoylethanolamide inhibits prostaglandin and hydroxyeicosatetraenoic acid production by a macrophage cell line.Pharmacology research & perspectives · 2017Article
- Activation mode of the eukaryotic m2G10 tRNA methyltransferase Trm11 by its partner protein Trm112.Nucleic acids research · 2017Article
- Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins.Journal of visualized experiments : JoVE · 2017Article
- Primer release is the rate-limiting event in lagging-strand synthesis mediated by the T7 replisome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Insights into molecular plasticity in protein complexes from Trm9-Trm112 tRNA modifying enzyme crystal structure.Nucleic acids research · 2015Article
- ENPP1-Fc prevents mortality and vascular calcifications in rodent model of generalized arterial calcification of infancy.Nature communications · 2015Article
- The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enzyme inhibition due to the reversible binding of reaction products is common and underlies the origins of negative feedback inhibition in many metabolic and signaling pathways. Product inhibition generates non-linearity in steady-state time courses of enzyme activity, which limits the utility of well-established enzymology approaches developed under the assumption of irreversible product release. For more than a century, numerous attempts to find a mathematical solution for analysis of kinetic time courses with product inhibition have been put forth. However, no practical general method capable of extracting common enzymatic parameters from such non-linear time courses has been successfully developed. Here we present a simple and practical method of analysis capable of efficiently extracting steady-state enzyme kinetic parameters and product binding constants from non-linear kinetic time courses with product inhibition and/or substrate depletion. The method is general and applicable to all enzyme systems, independent of reaction schemes and pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.