ArticleJournal of industrial microbiology & biotechnology2013
Improvement of cell growth and L-lysine production by genetically modified Corynebacterium glutamicum during growth on molasses.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Evolving a New Efficient Mode of Fructose Utilization for Improved Bioproduction inFrontiers in bioengineering and biotechnology · 2021Article
- Toward fine-tuned metabolic networks in industrial microorganisms.Synthetic and systems biotechnology · 2020Article
- Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production from mixed sugar.Microbial cell factories · 2020Article
- Metabolic engineering of glucose uptake systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production.Journal of industrial microbiology & biotechnology · 2019Article
- Equilibrium of the intracellular redox state for improving cell growth and L-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping.Microbial cell factories · 2019Article
- QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast.Biotechnology for biofuels · 2019Article
- Expanding lysine industry: industrial biomanufacturing of lysine and its derivatives.Journal of industrial microbiology & biotechnology · 2018Review
- Rational modification of tricarboxylic acid cycle for improving L-lysine production in Corynebacterium glutamicum.Microbial cell factories · 2018Article
- Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method.Journal of industrial microbiology & biotechnology · 2016Article
- Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression.Journal of Zhejiang University. Science. B · 2016Review
- Production of L-ornithine from sucrose and molasses by recombinant Corynebacterium glutamicum.Folia microbiologica · 2015Article
- Succinate production from CO₂-grown microalgal biomass as carbon source using engineered Corynebacterium glutamicum through consolidated bioprocessing.Scientific reports · 2014Article
- Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production.BMC microbiology · 2014Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Fructose-1,6-bisphosphatase (FBPase) and fructokinase (ScrK) have important roles in regenerating glucose-6-phosphate in the pentose phosphate pathway (PPP), and thus increasing L-lysine production. This article focuses on the development of L-lysine high-producing strains by heterologous expression of FBPase gene fbp and ScrK gene scrK in C. glutamicum lysC (fbr) with molasses as the sole carbon source. Heterologous expression of fbp and scrK lead to a decrease of residual sugar in fermentation broth, and heterologous expression of scrK prevents the fructose efflux. Heterologous expression of fbp and scrK not only increases significantly the activity of corresponding enzymes but also improves cell growth during growth on molasses. FBPase activities are increased tenfold by heterologous expression of fbp, whereas the FBPase activity is only increase fourfold during co-expression of scrK and fbp. Compared with glucose, the DCW of heterologous expression strains are higher on molasses except co-expression of fbp and scrK strain. In addition, heterologous expression of fbp and scrK can strongly increase the L-lysine production with molasses as the sole carbon source. The highest increase (88.4 %) was observed for C. glutamicum lysC (fbr) pDXW-8-fbp-scrK, but the increase was also significant for C. glutamicum lysC (fbr) pDXW-8-fbp (47.2 %) and C. glutamicum lysC (fbr) pDXW-8-scrK (36.8 %). By-products, such as glycerol and dihydroxyacetone, are decreased by heterologous expression of fbp and scrK, whereas trehalose is only slightly increased. The strategy for enhancing L-lysine production by regeneration of glucose-6-phosphate in PPP may provide a reference to enhance the production of other amino acids during growth on molasses or starch.
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