ArticleVirology journal2013
High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.
Article in Virology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- High-resolution melting analysis for detection of nucleotide mutation markers in the polymerase-acidic (PA) gene of influenza virus that are associated with baloxavir marboxil resistance.Archives of virology · 2025Article
- RT-qPCR genotyping assays for differentiating Rift Valley fever phlebovirus strains.Journal of virological methods · 2023Article
- Detection of the Omicron SARS-CoV-2 Lineage and Its BA.1 Variant with Multiplex RT-qPCR.International journal of molecular sciences · 2022Article
- Article
- High-resolution melting curve analysis for infectious bronchitis virus strain differentiation.Veterinary world · 2020Article
- Characterization of influenza A(H1N1)pdm09 isolates of Peru using HRM, a post PCR molecular biology method.Bioinformation · 2019Article
- Facile and Cost-Effective Detection of Saxitoxin Exploiting Aptamer Structural Switching.Food technology and biotechnology · 2015Article
- Molecular diagnosis of respiratory viruses.Pathology · 2015Review
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh resolution melting analysis (HRM) is a rapid and cost-effective technique for the characterisation of PCR amplicons. Because the reverse genetics of segmented influenza A viruses allows the generation of numerous influenza A virus reassortants within a short time, methods for the rapid selection of the correct recombinants are very useful.
methodsPCR primer pairs covering the single nucleotide polymorphism (SNP) positions of two different influenza A H5N1 strains were designed. Reassortants of the two different H5N1 isolates were used as a model to prove the suitability of HRM for the selection of the correct recombinants. Furthermore, two different cycler instruments were compared.
resultsBoth cycler instruments generated comparable average melting peaks, which allowed the easy identification and selection of the correct cloned segments or reassorted viruses.
conclusionsHRM is a highly suitable method for the rapid and precise characterisation of cloned influenza A genomes.
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