ArticleCell journal2013
Comparison the effects of two monocyte isolation methods, plastic adherence and magnetic activated cell sorting methods, on phagocytic activity of generated dendritic cells.
Article in Cell journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
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- Eicosapentaenoic acid induces macrophage Mox polarization to prevent diabetic cardiomyopathy.EMBO reports · 2024Article
- Rapid screening and scaled manufacture of immunogenic virus-like particles in a tobacco BY-2 cell-free protein synthesis system.Frontiers in immunology · 2023Article
- Negative Magnetic Sorting Preserves the Functionality of Ex Vivo Cultivated Non-Adherent Human Monocytes.Biology · 2022Article
- Activation of cytotoxic T lymphocytes by self-differentiated myeloid-derived dendritic cells for killing breast cancer cells expressing folate receptor alpha protein.Bioengineered · 2022Article
- Observational
- Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Inflammatory phenotype modulation in the respiratory tract and systemic circulation of e-cigarette users: a pilot study.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Vitamin E and selenium improve mesenchymal stem cell conditioned media immunomodulatory effects.Stem cell investigation · 2021Article
- Recent Advances in Good Manufacturing Practice-Grade Generation of Dendritic Cells.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2020Review
- Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment.Molecular therapy. Methods & clinical development · 2020Review
- Monocyte isolation techniques significantly impact the phenotype of both isolated monocytes and derived macrophages in vitro.Immunology · 2020Article
- Article
- A protocol for rapid monocyte isolation and generation of singular human monocyte-derived dendritic cells.PloS one · 2020Article
- In vitro analysis of antigen induced T cell-monocyte conjugates by imaging flow cytometry.Journal of immunological methods · 2018Article
- Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Role of the hepcidin-ferroportin axis in pathogen-mediated intracellular iron sequestration in human phagocytic cells.Blood advances · 2018Article
- Monocytes isolated by positive and negative magnetic sorting techniques show different molecular characteristics and immunophenotypic behaviour.F1000Research · 2017Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveIt is believed that monocyte isolation methods and maturation factors affect the phenotypic and functional characteristics of resultant dendritic cells (DC). In the present study, we compared two monocyte isolation methods, including plastic adherence-dendritic cells (Adh-DC) and magnetic activated cell sorting- dendritic cells (MACS-DC), and their effects on phagocytic activity of differentiated immature DCs (immDCs). MATERIALS AND
methods: In this experimental study, immDCs were generated from plastic adherence and MACS isolated monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) in five days. The phagocytic activity of immDCs was analyzed by fluorescein isothiocyanate (FITC)-conjugated latex bead using flow cytometry. One way ANOVA test was used for statistical analysis of differences among experimental groups, including Adh-DC and MACS-DC groups.
resultsWe found that phagocytic activity of Adh-DC was higher than MACS-DC, whereas the mean fluorescence intensity (MFI) of phagocytic cells was higher in MACS-DC (p<0.05).
conclusion: We concluded that it would be important to consider phagocytosis parameters of generated DCs before making any decision about monocyte isolation methods to have fully functional DCs.
Indexed as
Identifiers
24027662PMC3769603What OpenQuestion holds
Registered trials
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