ArticleInternational journal of cancer2014
Expulsion of micronuclei containing amplified genes contributes to a decrease in double minute chromosomes from malignant tumor cells.
Article in International journal of cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Chromosomal instability in cancer: sources, consequences and new therapeutic opportunities.Signal transduction and targeted therapy · 2026Review
- Extrachromosomal DNA and cancer: function, formation, and clinical implications.Journal of experimental & clinical cancer research : CR · 2026Review
- Article
- Origin and Fate of Micronuclei on the Road to Chromoanagenesis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Micronuclei and Cancer.Cancer discovery · 2024Review
- Article
- Extrachromosomal circular DNA in colorectal cancer: biogenesis, function and potential as therapeutic target.Oncogene · 2023Review
- Guilt by association: EcDNA as a mobile transactivator in cancer.Trends in cancer · 2022Review
- Revisiting characteristics of oncogenic extrachromosomal DNA as mobile enhancers on neuroblastoma and glioma cancers.Cancer cell international · 2022Review
- HolistIC: leveraging Hi-C and whole genome shotgun sequencing for double minute chromosome discovery.Bioinformatics (Oxford, England) · 2022Article
- Extrachromosomal Circular DNA: A New Target in Cancer.Frontiers in oncology · 2022Review
- Tribbles Pseudokinases in Colorectal Cancer.Cancers · 2021Review
- Small ring has big potential: insights into extrachromosomal DNA in cancer.Cancer cell international · 2021Review
- Extrachromosomal Circular DNAs: Origin, formation and emerging function in Cancer.International journal of biological sciences · 2021Review
- [Amplification of Extrachromosomal Oncogene and Tumorigenesis and Development].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2020Review
- Molecular structure and evolution mechanism of two populations of double minutes in human colorectal cancer cells.Journal of cellular and molecular medicine · 2020Article
- Novel insights into extrachromosomal DNA: redefining the onco-drivers of tumor progression.Journal of experimental & clinical cancer research : CR · 2020Review
- Article
- Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.American journal of translational research · 2019Article
- Cell-Free eccDNAs: A New Type of Nucleic Acid Component for Liquid Biopsy?Molecular diagnosis & therapy · 2018Review
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18 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Double minute chromosomes (DMs) are a hallmark of gene amplification. The relationship between the formation of DMs and the amplification of DM-carried genes remains to be clarified. The human colorectal cancer cell line NCI-H716 and human malignant primitive neuroectodermal tumor cell line SK-PN-DW are known to contain many DMs. To examine the amplification of DM-carried genes in tumor cells, we performed Affymetrix SNP Array 6.0 analyses and verified the regions of amplification in NCI-H716 and SK-PN-DW tumor cells. We identified the amplification regions and the DM-carried genes that were amplified and overexpressed in tumor cells. Using RNA interference, we downregulated seven DM-carried genes, (NDUFB9, MTSS1, NSMCE2, TRIB1, FAM84B, MYC and FGFR2) individually and then investigated the formation of DMs, the amplification of the DM-carried genes, DNA damage and the physiological function of these genes. We found that suppressing the expression of DM-carried genes led to a decrease in the number of DMs and reduced the amplification of the DM-carried genes through the micronuclei expulsion of DMs from the tumor cells. We further detected an increase in the number of γH2AX foci in the knockdown cells, which provides a strong link between DNA damage and the loss of DMs. In addition, the loss of DMs and the reduced amplification and expression of the DM-carried genes resulted in a decrease in cell proliferation and invasion ability.
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