ArticleWorld journal of microbiology & biotechnology2010
Enhanced production of squalene in the thraustochytrid Aurantiochytrium mangrovei by medium optimization and treatment with terbinafine.
Article in World journal of microbiology & biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 83 citations in OpenAlex.
- Production of squalene and fatty acids by Thraustochytrium sp. RT2316-16: effects of dissolved oxygen and the medium composition.Bioresources and bioprocessing · 2025Article
- Article
- Microbial Squalene: A Sustainable Alternative for the Cosmetics and Pharmaceutical Industry - A Review.Engineering in life sciences · 2024Review
- The Nitrogen Content in the Fruiting Body and Mycelium ofBioengineering (Basel, Switzerland) · 2024Article
- Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems.Applied microbiology and biotechnology · 2024Article
- The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics.Marine drugs · 2023Review
- Bioprocess conditions and regulation factors to optimize squalene production in thraustochytrids.World journal of microbiology & biotechnology · 2023Review
- Alpha-Tocopherol Significantly Improved Squalene Production Yield ofAntioxidants (Basel, Switzerland) · 2023Article
- Article
- Elucidation of sterol biosynthesis pathway and its co-regulation with fatty acid biosynthesis in the oleaginous marine protistFrontiers in bioengineering and biotechnology · 2023Article
- Recent advances in the microbial production of squalene.World journal of microbiology & biotechnology · 2022Review
- Media Supplementation with Mannitol and Biotin Enhances Squalene Production ofMolecules (Basel, Switzerland) · 2022Article
- Uncovering the Bioactivity of Aurantiochytrium sp.: a Comparison of Extraction Methodologies.Marine biotechnology (New York, N.Y.) · 2022Article
- Optimal NaCl Medium Enhances Squalene Accumulation inFrontiers in microbiology · 2022Article
- Recent Progress in Microalgal Squalene Production and Its Cosmetic Application.Biotechnology and bioprocess engineering : BBE · 2022Review
- Bioprospecting potentials of moderately halophilic bacteria and the isolation of squalene producers from Kuwait sabkha.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Corrigendum: Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Article
- Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2019Review
- Improving squalene production by enhancing the NADPH/NADPBiotechnology for biofuels · 2019Article
- A review on commercial-scale high-value products that can be produced alongside cellulosic ethanol.Biotechnology for biofuels · 2019Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Squalene is an effective chemopreventive agent in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study is to increase the squalene content and yield in the thraustochytrid, Aurantiochytrium mangrovei FB3 through medium optimization and the treatment with terbinafine, an inhibitor of squalene monooxygenase in the sterol biosynthetic pathway. The highest biomass concentration of 21.2 g l(-1) was obtained at a glucose concentration of 60 g l(-1), while the highest specific growth rate of 0.077 h(-1) and the growth yield coefficient of 0.44 g g(-1) based on glucose were achieved at a lower glucose concentration (30 g l(-1)). The addition of terbinafine led to a slight inhibition of cell growth whereas an obvious increase in squalene content was observed at terbinafine concentrations of 10 and 100 mg l(-1), which corresponded to an increase of 36 and 40% in squalene content, respectively compared to the control. The addition of terbinafine was thus effective in inducing the accumulation of squalene in A. mangrovei. This study not only demonstrated the production potential of squalene by A. mangrovei, but also provided novel information on the accumulation effect of terbinafine on the biosynthesis of an essential intermediate involved in sterol metabolic pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.