ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2013
Mapping nonrecombining regions in barley using multicolor FISH.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Article
- Chromosome and Genome Diversity in the GenusPlants (Basel, Switzerland) · 2021Review
- The 3D Genome: From Structure to Function.International journal of molecular sciences · 2021Review
- The B chromosome of Sorghum purpureosericeum reveals the first pieces of its sequence.Journal of experimental botany · 2021Article
- Development of DNA Markers From Physically Mapped Loci inFrontiers in plant science · 2021Article
- The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Integration of Physical, Genetic, and Cytogenetic Mapping Data for Cellulose Synthase (Frontiers in plant science · 2017Article
- High-resolution tyramide-FISH mapping of markers tightly linked to the male-fertility restoration (Ms) locus of onion.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Article
- The utility of flow sorting to identify chromosomes carrying a single copy transgene in wheat.Plant methods · 2016Article
- High resolution physical mapping of single gene fragments on pachytene chromosome 4 and 7 of Rosa.BMC genetics · 2015Article
- High-throughput physical map anchoring via BAC-pool sequencing.BMC plant biology · 2015Article
- Fluorescence in situ hybridization and optical mapping to correct scaffold arrangement in the tomato genome.G3 (Bethesda, Md.) · 2014Article
- Development of a wheat single gene FISH map for analyzing homoeologous relationship and chromosomal rearrangements within the Triticeae.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
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Authors and funding
8 authors.
Funding
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Abstract
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on chromosomes. Out of the many uses, FISH facilitates construction of physical maps by ordering contigs of large-insert DNA clones, typically bacterial artificial chromosome (BAC) and establishing their orientation. This is important in genomic regions with low recombination frequency where genetic maps suffer from poor resolution. While BAC clones can be mapped directly by FISH in plants with small genomes, excess of repetitive DNA hampers this application in species with large genomes. Mapping single-copy sequences such as complementary DNA (cDNA) is an attractive alternative. Unfortunately, localization of single-copy sequences shorter than 10 kb remains a challenging task in plants. Here, we present a highly efficient FISH technique that enables unambiguous localization of single copy genes. We demonstrated its utility by mapping 13 out of 15 full-length cDNAs of variable length (2,127-3,400 bp), which were genetically defined to centromeric and pericentromeric regions of barley chromosome 7H. We showed that a region of 1.2 cM (0.7 %) on genetic map represented more than 40 % of the physical length of the chromosome. Surprisingly, all cDNA probes occasionally revealed hybridization signals on other chromosomes, indicating the presence of partially homologous sequences. We confirmed the order of 10 cDNA clones and suggested a different position for three cDNAs as compared to published genetic order. These results underline the need for alternative approaches such as FISH, which can resolve the order of markers in genomic regions where genetic mapping fails.
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