ArticleScientific reports2013
Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 82 citations in OpenAlex.
- Improving Activity and Stability of Candida boidinii Formate Dehydrogenase Through Rational Active Site Engineering.Chembiochem : a European journal of chemical biology · 2026Article
- Dynamic changes of volatile compounds during coffee-grounds beer fermentation with different yeast strains: An integrated HS-SPME-GC/MS, electronic tongue and electronic nose analysis.Food chemistry: X · 2025Article
- Fast growth and high-titer bioproduction from renewable formate via metal-dependent formate dehydrogenase in Escherichia coli.Nature communications · 2025Article
- Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase.Microbial cell factories · 2023Review
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Redox Biocatalysis: Quantitative Comparisons of Nicotinamide Cofactor Regeneration Methods.ChemSusChem · 2022Review
- Highly efficient biosynthesis of spermidine from L-homoserine and putrescine using an engineered Escherichia coli with NADPH self-sufficient system.Applied microbiology and biotechnology · 2022Article
- Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass inBiomolecules · 2022Review
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Purification and Characterization of (2R,3R)-2,3-Butanediol Dehydrogenase of the Human Pathogen Neisseria gonorrhoeae FA1090 Produced in Escherichia coli.Molecular biotechnology · 2021Article
- Plant Growth Promotion by Two Volatile Organic Compounds Emitted From the FungusFrontiers in plant science · 2021Article
- Efficient bioproduction of 5-aminolevulinic acid, a promising biostimulant and nutrient, from renewable bioresources by engineeredBiotechnology for biofuels · 2020Article
- Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.Microbial cell factories · 2019Article
- Effects of NADH Availability on 3-Phenyllactic Acid Production by Lactobacillus plantarum Expressing Formate Dehydrogenase.Current microbiology · 2019Article
- New approaches to NAD(P)H regeneration in the biosynthesis systems.World journal of microbiology & biotechnology · 2018Review
- Enhanced production of optical (RSC advances · 2018Article
- Redox cofactor engineering in industrial microorganisms: strategies, recent applications and future directions.Journal of industrial microbiology & biotechnology · 2018Review
- Efficient (3S)-Acetoin and (2S,3S)-2,3-Butanediol Production from meso-2,3-Butanediol Using Whole-Cell Biocatalysis.Molecules (Basel, Switzerland) · 2018Article
- Engineering redox homeostasis to develop efficient alcohol-producing microbial cell factories.Microbial cell factories · 2017Review
- Production of C2-C4 diols from renewable bioresources: new metabolic pathways and metabolic engineering strategies.Biotechnology for biofuels · 2017Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
(2S,3S)-2,3-Butanediol ((2S,3S)-2,3-BD) is a potentially valuable liquid fuel and an excellent building block in asymmetric synthesis. In this study, cofactor engineering was applied to improve the efficiency of (2S,3S)-2,3-BD production and simplify the product purification. Two NADH regeneration enzymes, glucose dehydrogenase and formate dehydrogenase (FDH), were introduced into Escherichia coli with 2,3-BD dehydrogenase, respectively. Introduction of FDH resulted in higher (2S,3S)-2,3-BD concentration, productivity and yield from diacetyl, and large increase in the intracellular NADH concentration. In fed-batch bioconversion, the final titer, productivity and yield of (2S,3S)-2,3-BD on diacetyl reached 31.7 g/L, 2.3 g/(L·h) and 89.8%, the highest level of (2S,3S)-2,3-BD production thus far. Moreover, cosubstrate formate was almost totally converted to carbon dioxide and no organic acids were produced. The biocatalytic process presented should be a promising route for biotechnological production of NADH-dependent microbial metabolites.
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