ArticleAMB Express2013
Biochemical and kinetic characterisation of a novel xylooligosaccharide-upregulated GH43 β-d-xylosidase/α-l-arabinofuranosidase (BXA43) from the probiotic Bifidobacterium animalis subsp. lactis BB-12.
Article in AMB Express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Genetic and enzymatic basis of xylooligosaccharide metabolism byGut microbes · 2026Article
- Apoplastic proteomic reveals Colletotrichum fructicola effector CfXyn11A recognized by tobacco and suppressed by pear in the apoplast.Molecular horticulture · 2025Article
- Characterization of a GH43 Bifunctional Glycosidase from EndophyticBiotech (Basel (Switzerland)) · 2025Article
- Heterologous expression and characterization of xylose-tolerant GH 43 family β-xylosidase/α-L-arabinofuranosidase fromFrontiers in bioengineering and biotechnology · 2025Article
- Unveiling metabolic pathways of selected plant-derived glycans byFrontiers in microbiology · 2024Article
- Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria.International journal of molecular sciences · 2022Article
- Hydrolysis of Arabinoxylo-oligosaccharides by α-L-Arabinofuranosidases and β-D-Xylosidase fromJournal of microbiology and biotechnology · 2022Article
- Characterization of a GH Family 43 β-Xylosidase Having a Novel Carbohydrate-binding Module fromJournal of applied glycoscience · 2022Article
- Novel xylan-degrading enzymes from polysaccharide utilizing loci of Prevotella copri DSM18205.Glycobiology · 2021Article
- Plant Glycan Metabolism by Bifidobacteria.Frontiers in microbiology · 2021Review
- Multiple Transporters and Glycoside Hydrolases Are Involved in Arabinoxylan-Derived Oligosaccharide Utilization in Bifidobacterium pseudocatenulatum.Applied and environmental microbiology · 2020Article
- Molecular Characterization, Regioselective and Synergistic Action of First Recombinant Type III α-L-arabinofuranosidase of Family 43 Glycoside Hydrolase (PsGH43_12) from Pseudopedobacter saltans.Molecular biotechnology · 2020Article
- Article
- Identification of a Key Enzyme for the Hydrolysis of β-(1→3)-Xylosyl Linkage in Red Alga Dulse Xylooligosaccharide fromMarine drugs · 2020Article
- Functional Expression and Characterization of Acetyl Xylan Esterases CE Family 7 fromJournal of microbiology and biotechnology · 2020Article
- Identification and characterization of GH11 xylanase and GH43 xylosidase from the chytridiomycetous fungus, Rhizophlyctis rosea.Applied microbiology and biotechnology · 2019Article
- Proteinaceous Molecules Mediating Bifidobacterium-Host Interactions.Frontiers in microbiology · 2016Review
- Gene expression of enzymes involved in utilization of xylooligosaccharides byBiotechnology, biotechnological equipment · 2014Article
- Elucidation of the molecular basis for arabinoxylan-debranching activity of a thermostable family GH62 α-l-arabinofuranosidase from Streptomyces thermoviolaceus.Applied and environmental microbiology · 2014Article
- Biochemical characterization of two thermostable xylanolytic enzymes encoded by a gene cluster of Caldicellulosiruptor owensensis.PloS one · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bifidobacterium animalis subsp. lactis BB-12 gene BIF_00092, assigned to encode a β-d-xylosidase (BXA43) of glycoside hydrolase family 43 (GH43), was cloned with a C-terminal His-tag and expressed in Escherichia coli. BXA43 was purified to homogeneity from the cell lysate and found to be a dual-specificity exo-hydrolase active on para-nitrophenyl-β-d-xylopyranoside (pNPX), para-nitrophenyl-α-L-arabinofuranoside (pNPA), β-(1 → 4)-xylopyranosyl oligomers (XOS) of degree of polymerisation (DP) 2-4, and birchwood xylan. A phylogenetic tree of the 92 characterised GH43 enzymes displayed five distinct groups (I - V) showing specificity differences. BXA43 belonged to group IV and had an activity ratio for pNPA:pNPX of 1:25. BXA43 was stable below 40°C and at pH 4.0-8.0 and showed maximum activity at pH 5.5 and 50°C. Km and kcat for pNPX were 15.6 ± 4.2 mM and 60.6 ± 10.8 s-1, respectively, and substrate inhibition became apparent above 18 mM pNPX. Similar kinetic parameters and catalytic efficiency values were reported for β-d-xylosidase (XynB3) from Geobacillus stearothermophilus T‒6 also belonging to group IV. The activity of BXA43 for xylooligosaccharides increased with the size and was 2.3 and 5.6 fold higher, respectively for xylobiose and xylotetraose compared to pNPX. BXA43 showed clearly metal inhibition for Zn2+ and Ag+, which is different to its close homologues. Multiple sequence alignment and homology modelling indicated that Arg505Tyr506 present in BXA43 are probably important for binding to xylotetraose at subsite +3 and occur only in GH43 from the Bifidobacterium genus.
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Registered trials
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