ArticleBiochemical and biophysical research communications2013
Transient light-induced intracellular oxidation revealed by redox biosensor.
Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 8 citations in OpenAlex.
- Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in AβACS sensors · 2022Article
- Design considerations for open-well microfluidic platforms for hypoxic cell studies.Biomicrofluidics · 2017Article
- Thiol-based antioxidants elicit mitochondrial oxidation via respiratory complex III.American journal of physiology. Cell physiology · 2015Article
- Inhibition of glutathione synthesis distinctly alters mitochondrial and cytosolic redox poise.Experimental biology and medicine (Maywood, N.J.) · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have implemented a ratiometric, genetically encoded redox-sensitive green fluorescent protein fused to human glutaredoxin (Grx1-roGFP2) to monitor real time intracellular glutathione redox potentials of mammalian cells. This probe enabled detection of media-dependent oxidation of the cytosol triggered by short wavelength excitation. The transient nature of light-induced oxidation was revealed by time-lapse live cell imaging when time intervals of less than 30s were implemented. In contrast, transient ROS generation was not observed with the parental roGFP2 probe without Grx1, which exhibits slower thiol-disulfide exchange. These data demonstrate that the enhanced sensitivity of the Grx1-roGFP2 fusion protein enables the detection of short-lived ROS in living cells. The superior sensitivity of Grx1-roGFP2, however, also enhances responsiveness to environmental cues introducing a greater likelihood of false positive results during image acquisition.
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Registered trials
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