ArticleTissue engineering. Part A2014
Repopulation of intrasynovial flexor tendon allograft with bone marrow stromal cells: an ex vivo model.
Article in Tissue engineering. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Structure, ingredient, and function-based biomimetic scaffolds for accelerated healing of tendon-bone interface.Journal of orthopaedic translation · 2024Article
- Cell-based tissue engineered flexor tendon allograft: A canine in vivo study.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Pentamidine-loaded gelatin decreases adhesion formation of flexor tendon.Journal of orthopaedic translation · 2024Article
- Is there a duration-characteristic relationship for trypsin exposure on tendon? A study on anterior cruciate ligament reconstruction in a rabbit model.Frontiers in medicine · 2024Article
- Review
- Engineered Decellularized Tendon Matrix Putty Preserves Native Tendon Bioactivity to Promote Cell Proliferation and Enthesis Repair.Journal of tissue engineering and regenerative medicine · 2023Article
- Effect of Freshly Isolated Bone Marrow Mononuclear Cells and Cultured Bone Marrow Stromal Cells in Graft Cell Repopulation and Tendon-Bone Healing after Allograft Anterior Cruciate Ligament Reconstruction.International journal of molecular sciences · 2021Article
- Effect of book-shaped acellular tendon scaffold with bone marrow mesenchymal stem cells sheets on bone-tendon interface healing.Journal of orthopaedic translation · 2021Article
- Impact of Uniaxial Stretching on Both Gliding and Traction Areas of Tendon Explants in a Novel Bioreactor.International journal of molecular sciences · 2020Article
- Potential of Soluble Decellularized Extracellular Matrix for Musculoskeletal Tissue Engineering - Comparison of Various Mesenchymal Tissues.Frontiers in cell and developmental biology · 2020Article
- Lateral slit delivery of bone marrow stromal cells enhances regeneration in the decellularized allograft flexor tendon.Journal of orthopaedic translation · 2019Article
- Orthotopic Transplantation of Achilles Tendon Allograft in Rats: With or without Incorporation of Autologous Mesenchymal Stem Cells.Cell transplantation · 2018Article
- Tissue-specific bioactivity of soluble tendon-derived and cartilage-derived extracellular matrices on adult mesenchymal stem cells.Stem cell research & therapy · 2017Article
- Decellularized and Engineered Tendons as Biological Substitutes: A Critical Review.Stem cells international · 2016Review
- Biomechanical evaluation of flexor tendon graft with different repair techniques and graft surface modification.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2015Article
- Do cells contribute to tendon and ligament biomechanics?PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeDelayed healing is a common problem whenever tendon allografts are used for tendon or ligament reconstruction. Repopulating the allograft with host cells may accelerate tendon regeneration, but cell penetration into the allograft tendon is limited. Processing the tendon surface with slits that guide cells into the allograft substrate may improve healing. The purpose of this study was to describe a surface modification of allograft tendon that includes slits to aid cell repopulation and lubrication to enhance tendon gliding.
methodsCanine flexor digitorum profundus tendons were used for this study. Cyclic gliding resistance was measured over 1000 cycles. Tensile stiffness was assessed for normal tendon, tendon decellularized with trypsin and Triton X-100 (decellularized group), tendon decellularized and perforated with multiple slits (MS group) and tendon decellularized, perforated with slits and treated with a carbodiimide-derivatized hyaluronic acid and gelatin (cd-HA-gelatin) surface modification (MS-SM group). To assess tendon repopulation, bone marrow stromal cells (BMSCs) were used in the decellularized and MS groups. DNA concentration and histology were evaluated and compared to normal tendons and nonseeded decellularized tendons.
resultsThe gliding resistance of the decellularized and MS groups was significantly higher compared with the normal group. There was no significant difference in gliding resistance between the decellularized and MS group. Gliding resistance of the normal group and MS-SM group was not significantly different. The Young's modulus was not significantly different among the four groups. The DNA concentration in the MS group was significantly lower than in normal tendons, but significantly higher than in decellularized tendons, with or without BMSCs. Viable BMSCs were found in the slits after 2 weeks in tissue culture.
conclusionsTendon slits can successfully harbor BMSCs without compromising their survival and without changing tendon stiffness. Surface modification restores normal gliding function to the slit tendon. CLINICAL RELEVANCE: A multislit tendon reseeded with BMSCs, with a surface treatment applied to restore gliding properties, may potentially promote tendon revitalization and accelerate healing for tendon or ligament reconstruction applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.