ArticleSpringerPlus2013
Characterization of a new oxidant-stable serine protease isolated by functional metagenomics.
Article in SpringerPlus, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
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- Efficient Mining and Characterization of Two Novel Keratinases from Metagenomic Database.Biomolecules · 2025Article
- Effect of the Absence of α Carbonic Anhydrase 2 on the PSII Light-Harvesting Complex Size inPlants (Basel, Switzerland) · 2025Article
- Enzymes for production of whey protein hydrolysates and other value-added products.Applied microbiology and biotechnology · 2024Review
- Helping proteins come in from the cold: 5 burning questions about cold-active enzymes.BBA advances · 2024Review
- Reversible protein phosphorylation in higher plants: focus on state transitions.Biophysical reviews · 2023Review
- Purification and characterization of cysteine protease of Sarcocystis fusiformis from infected Egyptian water buffaloes.Scientific reports · 2023Article
- Optimization, partial purification, and characterization of a novel high molecular weight alkaline protease produced by Halobacillus sp. HAL1 using fish wastes as a substrate.Journal, genetic engineering & biotechnology · 2023Article
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- Two-step functional screen on multiple proteinaceous substrates reveals temperature-robust proteases with a broad-substrate range.Applied microbiology and biotechnology · 2021Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- Metagenomic applications in exploration and development of novel enzymes from nature: a review.Journal, genetic engineering & biotechnology · 2020Review
- Recovery and functional validation of hidden soil enzymes in metagenomic libraries.MicrobiologyOpen · 2019Article
- Recent Advances in Function-based Metagenomic Screening.Genomics, proteomics & bioinformatics · 2018Review
- A novel alkaline protease from alkaliphilic Idiomarina sp. C9-1 with potential application for eco-friendly enzymatic dehairing in the leather industry.Scientific reports · 2018Article
- Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes.Frontiers in microbiology · 2016Review
- Current Technological Improvements in Enzymes toward Their Biotechnological Applications.Frontiers in microbiology · 2016Review
- Isolation and in silico characterization of novel esterase gene with β-lactamase fold isolated from metagenome of north western Himalayas.3 Biotech · 2015Article
- Current functional metagenomic approaches only expand the existing protease sequence space, but does not presently add any novelty to it.Current microbiology · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel serine protease gene, SBcas3.3, was identified by functional screening of a forest-soil metagenomic library on agar plates supplemented with AZCL-casein. Overproduction in Escherichia coli revealed that the enzyme is produced as a 770-amino-acid precursor which is processed to a mature protease of ~55 kDa. The latter was purified by affinity chromatography for characterization with the azocasein substrate. The enzyme proved to be an alkaline protease showing maximal activity between pH 9 and 10 and at 50°C. Treatment with the chelating agent ethylenediaminetetraacetic acid irreversibly denatured the protease, whose stability was found to depend strictly on calcium ions. The enzyme appeared relatively resistant to denaturing and reducing agents, and its activity was enhanced in the presence of 10 ml/l nonionic detergent (Tween 20, Tween 80, or Triton X-100). Moreover, SBcas3.3 displayed oxidant stability, a feature particularly sought in the detergent and bleaching industries. SBcas3.3 was activated by hydrogen peroxide at concentrations up to 10 g/l and it still retained 30% of activity in 50 g/l H2O2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.