ArticlePloS one2013
Evaluating detection limits of next-generation sequencing for the surveillance and monitoring of international marine pests.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 1 synthesis or guideline pooled it.
- How quantitative is metabarcoding: A meta-analytical approach.Molecular ecology · 2019Pooled it
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- Integrating active and passive environmental DNA sampling into coral reef monitoring: implications for community-based stewardship in Hawai i.Research square · 2026Article
- Validating COI eDNA Metabarcoding Primers for Detection of Subterranean Fauna.Molecular ecology resources · 2026Article
- Swab vs rollers: a comparison of methods to collect eDNA from vegetative surfaces.BMC research notes · 2026Article
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Assessing the Seasonal and Spatial Dynamics of Zooplankton through DNA Metabarcoding in a Temperate Estuary.Animals : an open access journal from MDPI · 2023Article
- Chromosome-level genome assembly of the northern Pacific seastar Asterias amurensis.Scientific data · 2023Article
- An integrative protocol for one-step PCR amplicon library construction and accurate demultiplexing of pooled sequencing data.Marine life science & technology · 2023Article
- Harnessing environmental DNA to reveal biogeographical patterns of non-indigenous species for improved co-governance of the marine environment in Aotearoa New Zealand.Scientific reports · 2023Article
- Article
- Strategies for sample labelling and library preparation in DNA metabarcoding studies.Molecular ecology resources · 2022Review
- Article
- Assessing the Use of Molecular Barcoding and qPCR for Investigating the Ecology ofMicroorganisms · 2021Article
- Comparison of Larval Fish Detections Using Morphology-Based Taxonomy versus High-Throughput Sequencing for Invasive Species Early Detection.Canadian journal of fisheries and aquatic sciences. Journal canadien des sciences halieutiques et aquatiques · 2021Article
- Development of droplet digital Polymerase Chain Reaction assays for the detection of long-finned (PeerJ · 2021Article
- Under the karst: detecting hidden subterranean assemblages using eDNA metabarcoding in the caves of Christmas Island, Australia.Scientific reports · 2020Article
- Monitoring of biofouling communities in a Portuguese port using a combined morphological and metabarcoding approach.Scientific reports · 2020Article
- Environmental DNA can act as a biodiversity barometer of anthropogenic pressures in coastal ecosystems.Scientific reports · 2020Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most surveillance programmes for marine invasive species (MIS) require considerable taxonomic expertise, are laborious, and are unable to identify species at larval or juvenile stages. Therefore, marine pests may go undetected at the initial stages of incursions when population densities are low. In this study, we evaluated the ability of the benchtop GS Junior™ 454 pyrosequencing system to detect the presence of MIS in complex sample matrices. An initial in-silico evaluation of the mitochondrial cytochrome c oxidase subunit I (COI) and the nuclear small subunit ribosomal DNA (SSU) genes, found that multiple primer sets (targeting a ca. 400 base pair region) would be required to obtain species level identification within the COI gene. In contrast a single universal primer set was designed to target the V1-V3 region of SSU, allowing simultaneous PCR amplification of a wide taxonomic range of MIS. To evaluate the limits of detection of this method, artificial contrived communities (10 species from 5 taxonomic groups) were created using varying concentrations of known DNA samples and PCR products. Environmental samples (water and sediment) spiked with one or five 160 hr old Asterias amurensis larvae were also examined. Pyrosequencing was able to recover DNA/PCR products of individual species present at greater than 0.64% abundance from all tested contrived communities. Additionally, single A. amurensis larvae were detected from both water and sediment samples despite the co-occurrence of a large array of environmental eukaryotes, indicating an equivalent sensitivity to quantitative PCR. NGS technology has tremendous potential for the early detection of marine invasive species worldwide.
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