ArticlePloS one2013
Design and implementation of a custom built optical projection tomography system.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- iCAT as an open-source platform for axial rotation and high-resolution imaging of organoids and living organisms.Communications biology · 2025Article
- Quantifying the relationship between cell proliferation and morphology during development of the face.Development (Cambridge, England) · 2025Article
- A fibronectin gradient remodels mixed-phase mesoderm.Science advances · 2024Article
- An optimized workflow for microCT imaging of formalin-fixed and paraffin-embedded (FFPE) early equine embryos.Anatomia, histologia, embryologia · 2022Article
- Mouse embryo phenotyping using X-ray microCT.Frontiers in cell and developmental biology · 2022Review
- 3D imaging of human organs with micrometer resolution - applied to the endocrine pancreas.Communications biology · 2021Article
- Article
- NMIHBA results from hypomorphic PRUNE1 variants that lack short-chain exopolyphosphatase activity.Human molecular genetics · 2021Article
- Mesoscopic 3D imaging of pancreatic cancer and Langerhans islets based on tissue autofluorescence.Scientific reports · 2020Article
- IRX3/5 regulate mitotic chromatid segregation and limb bud shape.Development (Cambridge, England) · 2020Article
- zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging.Biomedical optics express · 2020Article
- Spatial mapping of tissue properties in vivo reveals a 3D stiffness gradient in the mouse limb bud.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Magnetic Micromanipulation forResearch (Washington, D.C.) · 2020Article
- OptiJ: Open-source optical projection tomography of large organ samples.Scientific reports · 2019Article
- High Resolution Imaging of Mouse Embryos and Neonates with X-Ray Micro-Computed Tomography.Current protocols in mouse biology · 2019Article
- Oscillatory cortical forces promote three dimensional cell intercalations that shape the murine mandibular arch.Nature communications · 2019Article
- A Label-free Multicolor Optical Surface Tomography (ALMOST) imaging method for nontransparent 3D samples.BMC biology · 2019Article
- Optical projection tomography for rapid whole mouse brain imaging.Biomedical optics express · 2017Article
- Genetic Disruption of 21-Hydroxylase in Zebrafish Causes Interrenal Hyperplasia.Endocrinology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Optical projection tomography (OPT) is an imaging modality that has, in the last decade, answered numerous biological questions owing to its ability to view gene expression in 3 dimensions (3D) at high resolution for samples up to several cm(3). This has increased demand for a cabinet OPT system, especially for mouse embryo phenotyping, for which OPT was primarily designed for. The Medical Research Council (MRC) Technology group (UK) released a commercial OPT system, constructed by Skyscan, called the Bioptonics OPT 3001 scanner that was installed in a limited number of locations. The Bioptonics system has been discontinued and currently there is no commercial OPT system available. Therefore, a few research institutions have built their own OPT system, choosing parts and a design specific to their biological applications. Some of these custom built OPT systems are preferred over the commercial Bioptonics system, as they provide improved performance based on stable translation and rotation stages and up to date CCD cameras coupled with objective lenses of high numerical aperture, increasing the resolution of the images. Here, we present a detailed description of a custom built OPT system that is robust and easy to build and install. Included is a hardware parts list, instructions for assembly, a description of the acquisition software and a free download site, and methods for calibration. The described OPT system can acquire a full 3D data set in 10 minutes at 6.7 micron isotropic resolution. The presented guide will hopefully increase adoption of OPT throughout the research community, for the OPT system described can be implemented by personnel with minimal expertise in optics or engineering who have access to a machine shop.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.