ArticlePloS one2013
The role of autophagy in chloroplast degradation and chlorophagy in immune defenses during Pst DC3000 (AvrRps4) infection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- Chloroplast Protein Degradation Pathways During Leaf Senescence: Coordination and Roles in Nitrogen Remobilization.Plants (Basel, Switzerland) · 2026Review
- The redox rhythm gates immune-induced cell death distinctly from the genetic clock.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Autophagy and Bacterial infections.Autophagy reports · 2025Review
- EMS-induced missense mutation in TaCHLI-7D affects leaf color and yield-related traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Vacuolar degradation of plant organelles.The Plant cell · 2024Review
- Autophagy formation, microtubule disorientation, and alteration of ATG8 and tubulin gene expression under simulated microgravity in Arabidopsis thaliana.NPJ microgravity · 2024Article
- Plant-Growth-Promoting Rhizobacteria Modulate Carbohydrate Metabolism in Connection with Host Plant Defense Mechanism.International journal of molecular sciences · 2024Review
- Mutation of a highly conserved amino acid in RPM1 causes leaf yellowing and premature senescence in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Chloroplast Envelopes Play a Role in the Formation of Autophagy-Related Structures in Plants.Plants (Basel, Switzerland) · 2023Article
- Cytoplasmic regulation of chloroplast ROS accumulation during effector-triggered immunity.Frontiers in plant science · 2023Review
- Comprehensive Expression Analyses of Plastidial Thioredoxins ofAntioxidants (Basel, Switzerland) · 2022Article
- Autophagic degradation of the chloroplastic 2-phosphoglycolate phosphatase TaPGLP1 in wheat.Plant cell reports · 2022Article
- A putative effector of the rubber-tree powdery mildew fungus has elicitor activity that can trigger plant immunity.Planta · 2022Article
- Functions of autophagy in chloroplast protein degradation and homeostasis.Frontiers in plant science · 2022Review
- The Small Secreted Protein FoSsp1 Elicits Plant Defenses and Negatively Regulates Pathogenesis inFrontiers in plant science · 2022Article
- Research Progress of ATGs Involved in Plant Immunity and NPR1 Metabolism.International journal of molecular sciences · 2021Review
- ATG4 MediatedInternational journal of molecular sciences · 2020Article
- Construction of gold-siRNARSC advances · 2020Article
- Combating stress: the interplay between hormone signaling and autophagy in plants.Journal of experimental botany · 2020Review
- A Candidate Secreted Effector Protein of Rubber Tree Powdery Mildew Fungus Contributes to Infection by Regulating Plant ABA Biosynthesis.Frontiers in microbiology · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundChlorosis of leaf tissue normally observed during pathogen infection may result from the degradation of chloroplasts. There is a growing evidence to suggest that the chloroplast plays a significant role during pathogen infection. Although most degradation of the organelles and cellular structures in plants is mediated by autophagy, its role in chloroplast catabolism during pathogen infection is largely unknown.
resultsIn this study, we investigated the function of autophagy in chloroplast degradation during avirulent Pst DC3000 (AvrRps4) infection. We examined the expression of defensive marker genes and suppression of bacterial growth using the electrolyte leakage assay in normal light (N) and low light (L) growing environments of wild-type and atg5-1 plants during pathogen treatment. Stroma-targeted GFP proteins (CT-GFP) were observed with LysoTracker Red (LTR) staining of autophagosome-like structures in the vacuole. The results showed that Arabidopsis expressed a significant number of small GFP-labeled bodies when infected with avirulent Pst DC3000 (AvrRps4). While barely detectable, there were small GFP-labeled bodies in plants with the CT-GFP expressing atg5-1 mutation. The results showed that chloroplast degradation depends on autophagy and this may play an important role in inhibiting pathogen growth.
conclusionAutophagy plays a role in chloroplast degradation in Arabidopsis during avirulent Pst DC3000 (AvrRps4) infection. Autophagy dependent chloroplast degradation may be the primary source of reactive oxygen species (ROS) as well as the pathogen-response signaling molecules that induce the defense response.
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