ArticlePloS one2013
PhagoSight: an open-source MATLAB® package for the analysis of fluorescent neutrophil and macrophage migration in a zebrafish model.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- An automated cell-tracking pipeline for the analysis of neutrophil dynamics.Frontiers in bioinformatics · 2026Article
- DMNet: Dual-Stream Marker Guided Deep Network for Dense Cell Segmentation and Lineage Tracking.... IEEE International Conference on Computer Vision workshops. IEEE International Conference on Computer Vision · 2021Article
- Cell Tracking Profiler - a user-driven analysis framework for evaluating 4D live-cell imaging data.Journal of cell science · 2020Article
- Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.Cell reports · 2020Article
- Comparative Study of Contact Repulsion in Control and Mutant Macrophages Using a Novel Interaction Detection.Journal of imaging · 2020Article
- Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart.Nature communications · 2019Article
- Segmentation and Modelling of the Nuclear Envelope of HeLa Cells Imaged with Serial Block Face Scanning Electron Microscopy.Journal of imaging · 2019Article
- β-glucan-dependent shuttling of conidia from neutrophils to macrophages occurs during fungal infection establishment.PLoS biology · 2019Article
- Visualisation and Analysis of Speech Production with Electropalatography.Journal of imaging · 2019Article
- Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using MatlabJournal of imaging · 2019Article
- An automated quantitative image analysis pipeline ofJournal of biological methods · 2018Article
- Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae.Journal of visualized experiments : JoVE · 2017Article
- An objective comparison of cell-tracking algorithms.Nature methods · 2017Article
- High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells.Journal of visualized experiments : JoVE · 2016Article
- Triple co-culture and perfusion bioreactor for studying the interaction betweenJournal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
Abstract
Neutrophil migration in zebrafish larvae is increasingly used as a model to study the response of these leukocytes to different determinants of the cellular inflammatory response. However, it remains challenging to extract comprehensive information describing the behaviour of neutrophils from the multi-dimensional data sets acquired with widefield or confocal microscopes. Here, we describe PhagoSight, an open-source software package for the segmentation, tracking and visualisation of migrating phagocytes in three dimensions. The algorithms in PhagoSight extract a large number of measurements that summarise the behaviour of neutrophils, but that could potentially be applied to any moving fluorescent cells. To derive a useful panel of variables quantifying aspects of neutrophil migratory behaviour, and to demonstrate the utility of PhagoSight, we evaluated changes in the volume of migrating neutrophils. Cell volume increased as neutrophils migrated towards the wound region of injured zebrafish. PhagoSight is openly available as MATLAB® m-files under the GNU General Public License. Synthetic data sets and a comprehensive user manual are available from http://www.phagosight.org.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.