ArticleEvolutionary bioinformatics online2013
Analysis of protein thermostability enhancing factors in industrially important thermus bacteria species.
Article in Evolutionary bioinformatics online, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- DDGWizard: Integration of feature calculation resources for analysis and prediction of changes in protein thermostability upon point mutations.PLoS computational biology · 2025Article
- Precision Thermostability Predictions: Leveraging Machine Learning for Examining Laccases and Their Associated Genes.International journal of molecular sciences · 2024Article
- The structural insights of L-asparaginase from3 Biotech · 2024Article
- Characterization of Molecular Diversity and Organization of Phycobilisomes in Thermophilic Cyanobacteria.International journal of molecular sciences · 2023Article
- A Statistical Analysis of the Sequence and Structure of Thermophilic and Non-Thermophilic Proteins.International journal of molecular sciences · 2022Article
- Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya.BMC microbiology · 2020Article
- Creation of a functional hyperthermostable designer cellulosome.Biotechnology for biofuels · 2019Article
- Functional Properties of Amino Acid Side Chains as Biomarkers of Extraterrestrial Life.Astrobiology · 2018Article
- The Effects of One Amino Acid Substitutions at the C-Terminal Region of Thermostable L2 Lipase by Computational and Experimental Approach.Molecular biotechnology · 2018Article
- Article
- Adaptive thermostability of light-harvesting complexes in marine picocyanobacteria.The ISME journal · 2017Article
- Truncation of the unique N-terminal domain improved the thermos-stability and specific activity of alkaline α-amylase Amy703.Scientific reports · 2016Article
- Article
- Mining the entire Protein DataBank for frequent spatially cohesive amino acid patterns.BioData mining · 2015Article
- Analysis of genomic rearrangements, horizontal gene transfer and role of plasmids in the evolution of industrial important Thermus species.BMC genomics · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Elucidation of evolutionary factors that enhance protein thermostability is a critical problem and was the focus of this work on Thermus species. Pairs of orthologous sequences of T. scotoductus SA-01 and T. thermophilus HB27, with the largest negative minimum folding energy (MFE) as predicted by the UNAFold algorithm, were statistically analyzed. Favored substitutions of amino acids residues and their properties were determined. Substitutions were analyzed in modeled protein structures to determine their locations and contribution to energy differences using PyMOL and FoldX programs respectively. Dominant trends in amino acid substitutions consistent with differences in thermostability between orthologous sequences were observed. T. thermophilus thermophilic proteins showed an increase in non-polar, tiny, and charged amino acids. An abundance of alanine substituted by serine and threonine, as well as arginine substituted by glutamine and lysine was observed in T. thermophilus HB27. Structural comparison showed that stabilizing mutations occurred on surfaces and loops in protein structures.
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