ArticleActa histochemica et cytochemica2013
Reassembly of anterior pituitary organization by hanging drop three-dimensional cell culture.
Article in Acta histochemica et cytochemica, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Emerging human cell-based in vitro models for studying pituitary development and disorders.Endocrine journal · 2026Review
- Endocrine cancer organoids in basic and translational medical research.Science China. Life sciences · 2025Review
- CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.Cell and tissue research · 2025Article
- Three-Dimensional Culture of Glioblastoma Cells Using a Tissueoid Cell Culture System.Acta histochemica et cytochemica · 2024Article
- Three-dimensional cell culture using CD9-positive cells isolated from marginal cell layer of intermediate lobe of rats sustains in vivo-like primary niche environment.The Journal of reproduction and development · 2024Article
- Organoid models of the pituitary gland in health and disease.Frontiers in endocrinology · 2023Review
- Article
- Expression and localization of tight junction-related proteins in adult rat pituitary stem/progenitor cell niches.The Journal of reproduction and development · 2022Article
- Three Dimensional Cell Culturing for Modeling Adrenal and Pituitary Tumors.Pathology oncology research : POR · 2021Article
- Unconventional Actions of Glycoprotein Hormone Subunits: A Comprehensive Review.Frontiers in endocrinology · 2021Review
- Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs.Frontiers in endocrinology · 2019Article
- Article
- Fibromodulin Expression in Folliculostellate Cells and Pericytes Is Promoted by TGFβ Signaling in Rat Anterior Pituitary Gland.Acta histochemica et cytochemica · 2016Article
- State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology.ALTEX · 2014Review
- Folliculostellate cells are required for laminin release from gonadotrophs in rat anterior pituitary.Acta histochemica et cytochemica · 2014Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The anterior pituitary gland comprises 5 types of hormone-producing cells and non-endocrine cells, such as folliculostellate (FS) cells. The cells form a lobular structure surrounded by extracellular matrix (ECM) but are not randomly distributed in each lobule; hormone-producing cells have affinities for specific cell types (topographic affinity), and FS cells form a homotypic meshwork. To determine whether this cell and ECM organization can be reproduced in vitro, we developed a 3-dimensional (3D) model that utilizes hanging drop cell culture. We found that the topographic affinities of hormone-producing cells were indeed maintained (ie, GH to ACTH cells, GH to TSH cells, PRL to LH/FSH cells). Fine structures in hormone-producing cells retained their normal appearance. In addition, FS cells displayed well-developed cytoplasmic protrusions, which interconnected with adjacent FS cells to form a 3D meshwork. In addition, reassembly of gap junctions and pseudofollicles among FS cells was observed in cell aggregates. Major ECM components-collagens and laminin-were deposited and distributed around the cells. In sum, the dissociated anterior pituitary cells largely maintained their in vivo anterior pituitary architectures. This culture system appears to be a powerful experimental tool for detailed analysis of anterior pituitary cell organization.
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