ArticlePflugers Archiv : European journal of physiology2014
Ion transporters in brain endothelial cells that contribute to formation of brain interstitial fluid.
Article in Pflugers Archiv : European journal of physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- The myth of the metabolic baseline: sleep-wake cycles undermine a foundational assumption in organismal biology.Biological reviews of the Cambridge Philosophical Society · 2026Review
- The ion transport, GPCR, and RTK toolkit expression in the human cerebrovascular endothelial cell line, hCMEC/D3: an Omics perspective.Frontiers in physiology · 2025Article
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- Dysregulation of Ion Channels and Transporters and Blood-Brain Barrier Dysfunction in Alzheimer's Disease and Vascular Dementia.Aging and disease · 2024Review
- Alterations in brain fluid physiology during the early stages of development of ischaemic oedema.Fluids and barriers of the CNS · 2024Review
- Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature.Brain, behavior, & immunity - health · 2023Article
- Inhibition of HMGB1 Attenuates Spinal Cord Edema by Reducing the Expression of NaJournal of neurotrauma · 2023Article
- Retrospective Analysis of Transnasal Endoscopic CSF Rhinorrhea Repair in a Tertiary Care Centre.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2022Article
- Hypotension in hereditary cardiomyopathy.Pflugers Archiv : European journal of physiology · 2022Article
- Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited.Fluids and barriers of the CNS · 2022Review
- Key brain cell interactions and contributions to the pathogenesis of Alzheimer's disease.Frontiers in cell and developmental biology · 2022Review
- Article
- Molecular mechanisms of brain water transport.Nature reviews. Neuroscience · 2021Review
- Blood-Brain Barrier Overview: Structural and Functional Correlation.Neural plasticity · 2021Review
- The New Frontiers in Neurodegenerative Diseases Treatment: Liposomal-Based Strategies.Frontiers in bioengineering and biotechnology · 2020Review
- Brain tumor acidification using drugs simultaneously targeting multiple pH regulatory mechanisms.Journal of neuro-oncology · 2019Article
- Approaches to CNS Drug Delivery with a Focus on Transporter-Mediated Transcytosis.International journal of molecular sciences · 2019Review
- In vivo detection of acute intracellular acidification in glioblastoma multiforme following a single dose of cariporide.International journal of clinical oncology · 2018Article
- Fluid outflow in the rat spinal cord: the role of perivascular and paravascular pathways.Fluids and barriers of the CNS · 2018Article
- Blood-brain barrier breakdown in Alzheimer disease and other neurodegenerative disorders.Nature reviews. Neurology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Ions and water transported across the endothelium lining the blood–brain barrier contribute to the fluid secreted into the brain and are important in maintaining appropriate volume and ionic composition of brain interstitial fluid. Changes in this secretion process may occur after stroke. The present study identifies at transcript and protein level ion transporters involved in the movement of key ions and examines how levels of certain of these alter following oxidative stress. Immunohistochemistry provides evidence for Cl−/HCO3− exchanger, AE2, and Na+, HCO3− cotransporters, NBCe1 and NBCn1, on brain microvessels. mRNA analysis by RT-PCR reveals expression of these transporters in cultured rat brain microvascular endothelial cells (both primary and immortalized GPNT cells) and also Na+/H+ exchangers, NHE1 (primary and immortalized) and NHE2 (primary cells only). Knock-down using siRNA in immortalized GPNT cells identifies AE2 as responsible for much of the Cl−/HCO3− exchange following extracellular chloride removal and NHE1 as the transporter that accounts for most of the Na+/H+ exchange following intracellular acidification. Transcript levels of both AE2 and NHE1 are increased following hypoxia/reoxygenation. Further work is now required to determine the localization of the bicarbonate transporters to luminal or abluminal membranes of the endothelial cells as well as to identify and localize additional transport mechanisms that must exist for K+ and Cl−.
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Registered trials
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