ArticleThe Journal of biological chemistry2013
Silk gland factor-2, involved in fibroin gene transcription, consists of LIM homeodomain, LIM-interacting, and single-stranded DNA-binding proteins.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 60 citations in OpenAlex.
- Targeting the circadian negative regulatorMaterials today. Bio · 2026Article
- Characterization ofInsects · 2026Article
- Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors inInsects · 2022Article
- New insights into the proteins interacting with the promoters of silkworm fibroin genes.Scientific reports · 2021Article
- Reference Transcriptome Data in SilkwormInsects · 2021Article
- Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori.PloS one · 2021Article
- Transgenic Ectopic Overexpression of Broad Complex (Insects · 2020Article
- Genome of the webworm Hyphantria cunea unveils genetic adaptations supporting its rapid invasion and spread.BMC genomics · 2020Article
- Comparative mRNA and LncRNA Analysis of the Molecular Mechanisms Associated With Low Silk Production inFrontiers in genetics · 2020Article
- Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system.Transgenic research · 2019Article
- Deep Insight into the Transcriptome of the Single Silk Gland ofInternational journal of molecular sciences · 2019Article
- New insight into the mechanism underlying the silk gland biological process by knocking out fibroin heavy chain in the silkworm.BMC genomics · 2018Article
- Genome-wide open chromatin regions and their effects on the regulation of silk protein genes in Bombyx mori.Scientific reports · 2017Article
- Regulation of Silk Genes by Hox and Homeodomain Proteins in the Terminal Differentiated Silk Gland of the Silkworm Bombyx mori.Journal of developmental biology · 2016Review
- A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori.The Journal of biological chemistry · 2016Article
- A juvenile hormone transcription factor Bmdimm-fibroin H chain pathway is involved in the synthesis of silk protein in silkworm, Bombyx mori.The Journal of biological chemistry · 2015Article
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11 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
SGF-2 binds to promoter elements governing posterior silk gland-specific expression of the fibroin gene in Bombyx mori. We purified SGF-2 and showed that SGF-2 contains at least four gene products: the silkworm orthologues of LIM homeodomain protein Awh, LIM domain-binding protein (Ldb), a sequence-specific single-stranded DNA-binding protein (Lcaf), and the silk protein P25/fibrohexamerin (fhx). Using co-expression of these factors in Sf9 cells, Awh, Ldb, and Lcaf proteins were co-purified as a ternary complex that bound to the enhancer sequence in vitro. Lcaf interacts with Ldb as well as Awh through the conserved regions to mediate transcriptional activation in yeast. Misexpression of Awh in transgenic silkworms induces ectopic expression of the fibroin gene in the middle silk glands, where Ldb and Lcaf are expressed. Taken together, this study demonstrates that SGF-2 is a multisubunit activator complex containing Awh. Moreover, our results suggest that the Ldb·Lcaf protein complex serves as a scaffold to facilitate communication between transcriptional control elements.
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