ArticleJournal of visualized experiments : JoVE2013
Improving the success rate of protein crystallization by random microseed matrix screening.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- Biophysical and Structural Characterization of Novel RAS-Binding Domains (RBDs) of PI3Kα and PI3Kγ.Journal of molecular biology · 2021Article
- Inhibition, crystal structures, and in-solution oligomeric structure of aldehyde dehydrogenase 9A1.Archives of biochemistry and biophysics · 2020Article
- Structural analysis of pathogenic mutations targeting Glu427 of ALDH7A1, the hot spot residue of pyridoxine-dependent epilepsy.Journal of inherited metabolic disease · 2020Article
- Macrophage migration inhibitory factor of Syrian golden hamster shares structural and functional similarity with human counterpart and promotes pancreatic cancer.Scientific reports · 2019Article
- Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.Journal of visualized experiments : JoVE · 2018Article
- Characterizing metal-binding sites in proteins with X-ray crystallography.Nature protocols · 2018Article
- Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology.Journal of visualized experiments : JoVE · 2018Article
- Regulators of complement activity mediate inhibitory mechanisms through a common C3b-binding mode.The EMBO journal · 2016Article
- Crystallographic analysis of the N-terminal domain of Middle East respiratory syndrome coronavirus nucleocapsid protein.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Automation in biological crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.
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