ArticleCalcified tissue international2013
Osteoclasts on bone and dentin in vitro: mechanism of trail formation and comparison of resorption behavior.
Article in Calcified tissue international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 3 of them syntheses that pooled it.
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Who cites it
43 citing papers in PubMed, 3 syntheses or guidelines pooled it, 66 citations in OpenAlex.
- Extracellular Vesicles for Treatment of Bone Resorption-Related Diseases in Animal Models: Systematic Review.Calcified tissue international · 2026Pooled it
- Pooled it
- Osteoblast-osteoclast co-cultures: A systematic review and map of available literature.PloS one · 2021Pooled it
- In vivo effects of different orthodontic loading on root resorption and correlation with mechanobiological stimulus in periodontal ligament.Journal of the Royal Society, Interface · 2019Trial
- Rapid generation of dental pulp stem cell-derived mineralized extracellular matrix for quantitative osteoclast resorption assays.Frontiers in bioengineering and biotechnology · 2026Article
- An in vivo assay for osteoclast activity using mouse calvaria.Animal models and experimental medicine · 2026Article
- Hypergravity reduces F-actin accumulation in osteoclasts, with attenuated bone resorption.PloS one · 2026Article
- Microdamage in biological hard tissues and its repair mechanisms.Biomedical engineering online · 2025Review
- Cathepsin K inhibitors promote osteoclast-osteoblast communication and engagement of osteogenesis.JBMR plus · 2025Article
- Bone, dentin and cementum differentially influence the differentiation of osteoclast-like cells.Scientific reports · 2025Article
- Six-Year Implants Follow-Up After Guided Bone Regeneration Using Autologous Tooth Graft: Innovative Biomaterial for Bone Regeneration Tooth TransformerJournal of functional biomaterials · 2025Article
- Identification of anti-resorptive GPCRs by high-content imaging in human osteoclasts.Journal of molecular endocrinology · 2025Article
- An In Vitro Model of Murine Osteoclast-Mediated Bone Resorption.Bio-protocol · 2024Article
- Article
- Transcriptional reprogramming during human osteoclast differentiation identifies regulators of osteoclast activity.Bone research · 2024Article
- A Machine Learning-Based Image Segmentation Method to Quantify In Vitro Osteoclast Culture Endpoints.Calcified tissue international · 2023Article
- Transcriptomic Differences Underlying the Activin-A Induced Large Osteoclast Formation in Both Healthy Control and Fibrodysplasia Ossificans Progressiva Osteoclasts.International journal of molecular sciences · 2023Article
- Effect of Gamma Irradiation on the Osteoinductivity of Demineralized Dentin Matrix for Allografts: A Preliminary Study.Journal of functional biomaterials · 2022Article
- Dihydroartemisinin attenuates osteoclast formation and bone resorption via inhibiting the NF‑κB, MAPK and NFATc1 signaling pathways and alleviates osteoarthritis.International journal of molecular medicine · 2022Article
- Dual protective role of velutin against articular cartilage degeneration and subchondral bone lossFrontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
The main function of osteoclasts in vivo is the resorption of bone matrix, leaving behind typical resorption traces consisting of pits and trails. The mechanism of pit formation is well described, but less is known about trail formation. Pit-forming osteoclasts possess round actin rings. In this study we show that trail-forming osteoclasts have crescent-shaped actin rings and provide a model that describes the detailed mechanism. To generate a trail, the actin ring of the resorption organelle attaches with one side outside the existing trail margin. The other side of the ring attaches to the wall inside the trail, thus sealing that narrow part to be resorbed next (3–21 lm). This 3D configuration allows vertical resorption layer-by-layer from the surface to a depth in combination with horizontal cell movement. Thus, trails are not just traces of a horizontal translation of osteoclasts during resorption. Additionally, we compared osteoclastic resorption on bone and dentin since the latter is the most frequently used in vitro model and data are extrapolated to bone. Histomorphometric analyses revealed a material-dependent effect reflected by an 11-fold higher resorption area and a sevenfold higher number of pits per square centimeter on dentin compared to bone. An important material-independent aspect was reflected by comparable mean pit area (μm²) and podosome patterns. Hence, dentin promotes the generation of resorbing osteoclasts, but once resorption has started, it proceeds independently of material properties. Thus, dentin is a suitable model substrate for data acquisition as long as osteoclast generation is not part of the analyses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.